摘要
目的研究视神经损伤对兴奋性氨基酸转运蛋白-1(EAAT-1)的表达以及对视网膜谷氨酸(Glu)摄取的影响。方法建立大鼠右眼视神经损伤模型48只。术后1、7、14 d以高效液相色谱分析检测大鼠玻璃体谷氨酸浓度;通过免疫组织化学检测大鼠视网膜EAAT-1的表达。结果视神经损伤后1、7、14 d,实验眼玻璃体谷氨酸浓度升高,与对照眼比较,差异均有统计学意义(t1 h=10.171,P=0.000;t7 h=3.871,P=0.006;t14 h=5.599,P=0.001);实验眼视网膜EAAT-1表达降低,与对照眼比较,差异均有统计学意义(t1 h=3.460,P=0.011;t7 h=4.182,P=0.004;t14 h=4.077,P=0.005)。结论视神经损伤后视网膜内谷氨酸积聚,与视网膜EAAT-1的表达降低有关。
Objective The high concentration of glutamate in intercellular matrix of retina induce the apoptosis of retinal ganglion cells by playing the excitotoxicity. Many researches and our previous study also showed that injury nf optical nerve,ocular hypertention and ischemia of retina results in the increase of glutamate concentration in vitreous. The aim of this study was to investigate the expression of excitatory amino acid transporter-1 （ EAAT-1 ） in rat retina after optical nerve contusion. Methods The model of optical nerve contusion was established in forty-eight Sprauge-Dawley（SD） rats by clipping exposure optical nerve for 30 seconds with artery forceps in the right eyes as experimental eyes,and the optical nerves of left eyes were not clipped as sham control group. Vitreous samples were collected from 24 rats at 1 day, 7, 14 days after contusion, and the glutamate concentration in vitreous was detected by high-performance liquid chromatography. The chromatographic peak area was recommended as the calculating index of glutamate concentration. Eyeballs of other 24 rats were enucleated at above time points for the detection of expression of EAAT-1 by immunohistochemistry,and the expression of EAAT-1 was quantitated using mean gray scale. Results The vitreous glutamate concentration in the experimental eyes was higher than that of the sham eyes at 1 day,7,14 days after contusion （t1b = 10. 171,P = 0.000;t7h = 3.871 ,P = 0.006; t14h = 5. 599, P = 0.001 ）. However, the expression level of EAAT-1 was significantly lower in experimental eyes than that of the sham eyes at 1 day,7,14 days after contusion（t1h=3, 460,P=0. 011;t7h =4. 182,P =0.004;t14b, =4.077,P =0.005）. Conclusion Optical nerve contusion could down-regulate the expression of EAAT-1 in retina,which results in glutamate accumulation.
出处
《眼科研究》
CSCD
北大核心
2009年第10期884-887,共4页
Chinese Ophthalmic Research
