摘要
目的建立一种慢性青光眼模型,为青光眼的基础和临床科研提供实验条件。方法使用波长532nm的氪离子黄光通过角膜缘光凝20只Wistar大鼠的右眼小梁网,左眼为对照眼。分别于光凝前,光凝后不同时间测量眼压,检测并比较视网膜神经节细胞(RGCs)的丢失程度。结果第1次激光光凝后3d,第2次光凝后3、7、14、28、35d,实验眼光凝后不同时间眼压较光凝前明显升高(P<0.01)。第2次光凝后35d光凝眼视网膜铺片RGCs平均密度为(1992±108)个/mm2,对照眼RGCs平均密度为(2516±196)个/mm2,光凝眼较对照眼明显减少(P<0.01)。结论激光光凝损伤小梁网引起眼压升高是建立慢性青光眼模型的一种简单有效的方法。
Objective To establish a simple, economic and practical chronic experimental glaucoma model has an important significance in the basic study and clinical research of glaucoma. The purpose of this paper was to creat a chronic glaucoma model by laser irradiating trabecular meshwork （TM） via external limbus and assess the damage of retinal ganglion cells. Methods Krypton laser was used to target at the TM in the right eyes of 20 Wistar rats with the wavelength 532 nm, light spot diameter 200μm,duration 0.7 s and power 0. 4 W. The left eyes without treatment were as control group. 80 - 100 spots were set along the 360° of eyeball for 2 times at a 7-day interval. Intraocular pressure（IOP） was measured using a tonometer at different times following photocoagulation of laser. The average density of retinal ganglion cells（RGCs） in photocoagulated eyes and control eyes was compared. Results IOP in the photocoagulated eyes were （25.30±9.40） , （27.15 ± 6.02） , （26. 12 ± 8.63） ,（26.20 ±2.69） , （26. 15 ±3.29） and （25.55 ± 1.20） mmHg at 3 days after the first laser and at 3,7,14,28,35 days after the second laser respectively. IOP in the control eyes were （ 12.25 ± 3.54） , （ 12.30 ± 3.48） , （ 12. 28 ± 3.76） , （ 12.14 ± 3.53） , （ 12.36 ± 3.72） and （ 12.60 ± 3.49） mmHg, showing that IOP of experimental eyes at various times after laser were significantly higher than that before laser （ P 〈 0. 01 ）. The average density of RGCs in the photocoagulated eyes was （1 992 ± 108） cells/mm^2,and that of the control eyes was （2 516 ± 196） cells/mm^2, indicating a significantly lower density value in the photocoagulated eyes in comparison with control eyes at 35 days after the second irradiation （ P 〈 0. 01 ）. Conclusion Photocoagulation of TM with translimbal laser to elevate the IOP is a simple and an effective method of establishing chronic experimental glaucoma model.
出处
《眼科研究》
CAS
CSCD
北大核心
2007年第2期102-104,共3页
Chinese Ophthalmic Research
关键词
氪激光
光凝
慢性青光眼模型
眼压
krypton laser
photocoagulation
chronic experimental glaucoma model
intraocular pressure
