摘要
采用光学显微镜和电子显微镜对冷冻前后的梅花鹿精子的形态和超显微结构进行了观察。结果表明：梅花鹿精子全长６１．６±２．７０μｍ，其中头长７．１９±０．４７μｍ，中段长１２．０８±０．７５μｍ。线粒体的螺旋数是６３．６８±４．６６旋，中段线粒体每个螺旋约由３～５个线粒体组成。梅花鹿精子超微结构有３个特点：一是头部的厚度为牛、羊、猪精子的１／２；二是在中环处的质膜未见反折现象，并且主段与中段的联接是以套管式镶嵌；三是末段以９＋２结构变成２０根（１２＋７＋１）单丝管形式排列。冷冻处理可使梅花鹿精子顶体膨胀，顶体内容物丢失，顶体内发生空泡，顶体外膜自身囊泡化，线粒体发生断裂和丢失，末段纤维束因质膜丢失而分散成扫帚状。冷冻后的梅花鹿精子畸形率极显著增高（Ｐ＜０．０１），冷冻解冻是致使梅花鹿精子顶体完整率和活力降低的主要原因。
The general morphology and ultrastructure of the spermatozoa of sika deer were observed and studied under light and electronic microscope before and after freezing.The results show that the length of whole sika deer sperm is 61.60±2.70μm,with head 7 19±0 47μm and middle part 12.08±0.75μm.Each mitochondrium has 63.68±4.66 helixes,and the helixes in the middle have 3～5 mitochondria. Comparatively there are three characters on the ultrastructure of sika deer spermatozoa:(1) Its head is only as half thick as that of bull,sheep and pigs;(2) The plasma membrane around the ammulus isnt reflected and its principal section is inlayed with the middle part;(3) The arrangement of microtubes in the end section undergoes a transition from 9+2 structure to 20 single microtubes in the pattern of 12+7+1. After freezing the morphology and ultrastructure of sika deer sperm chang a lot:its acrosomes become expanded and their content released;Both inside and outside the outer membrane of acrosomes becomes vesicularized;The mitochondria are broken or lost;the microtubes in the end section are extended like a broom because the plasma membrane lost.The rate of abnormal sperms increases significantly after freezing.Freezing and thawing is thought the main factors which cause reduction in the acrosomal integrity and the motality rate of sika deer sperm.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1998年第6期493-498,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
