摘要
目的探讨急性胆管炎大鼠血浆、胆汁中肿瘤坏死因子-α含量的变化。方法经胆总管间断小剂量注入大肠杆菌,建立大鼠急性胆管炎模型,用酶联免疫吸附测定(ELISA)方法分别检测在不同的时相点血浆和胆汁中肿瘤坏死因子-α(TNF-α)含量的变化。结果随时间延长大鼠死亡率增加,血浆和胆汁中TNF-α含量呈进行性升高,血清总胆红素升高。结论检测TNF-α的含量对评估急性胆管炎的发展、变化进程有一定意义。
Objective To investigate changes of Tumor Necrosis Factor-α（TNF-α）concentration in plasma and bile of rats with acute cholangitis. Methods Models of acute cholangitis in rats were created by the intermittent injection low-dose Escheriehia coli into common bile duct. Changes of TNF-α concentration in plasma and bile were detected with enzyme linked immunosorbent assay （ELISA） in different time points. Results With the extension of time,mortality of rats was increased,TNF-α concentration in plas- ma,bile,and the total serum bilirubin were progressive increased. Conclusion Detection of TNF-α for assessment of the develop- ment and process of acute cholangitis is a certain significance.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第20期2601-2602,共2页
Chongqing medicine
关键词
胆管炎
肿瘤坏死因子
大鼠
血浆
胆汁
acute cholangitis
tumor necrosis factor
rats
plasma
bile
