摘要
用乙型肝炎病毒(HBV)DNA转染的2215细胞株,对大黄挥发油体外抗HBV作用进行研究。同时应用四甲基偶氮唑盐(MTT)比色法检测药物对细胞的毒性。选用阿昔洛韦(无环鸟苷)作阳性对照药。结果发现大黄挥发油对细胞的半数毒性浓度(CD_(50))>0.125 g/L,其浓度在0.0625g/L以下时,细胞存活率在90％以上,对HBsAg和HBeAg的最大抑制率分别是70.71±5.4％和30.99±5.3％,表明大黄挥发油具有体外抗HBV作用。
The antiHBV effect of Rheum palmatum Volatile oil was studied by using 2215 cell line transfected with HBV DNA. At the same time MTT method was applied for the detection of cytoxicity of drugs, selecting acyclovir(ACV) as control medicine. It turns out that the toxic concentration of Rheum palmatum Volatile oil for 50% cells was (CD50) > 1.25 × 10-1g/L. When concentration was below 0.625 × 10-1g/L, the survival rate of cells was over 90% . The maximum inhibitory rates for HB-sAg and HBeAg were 70.71±5.4% and 30.99±5.3% respectively. This shows Rheum palmatum Volatile oil possesses the effect of antiHBV in vitro.
出处
《中药材》
CAS
CSCD
1998年第10期524-526,共3页
Journal of Chinese Medicinal Materials
