摘要
目的:建立花蕾期茵陈4个黄酮类成分的含量测定方法。方法:样品用醋酸乙酯超声提取30min。KromasilODS柱,流动相乙腈四氢呋喃 1%醋酸水溶液(2 5∶2 1. 5∶76 ) ,检测波长347nm。结果:茵陈花蕾中的4个黄酮类成分5 ,7,4′. 三羟基 6 ,2′,5′. 三甲氧基黄酮(arcapillin) ,5 ,3′,4′. 三羟基 6 ,7 .二甲氧基黄酮(cirsiliol) ,蓟黄素(cirsimaritin)和3′甲氧基蓟黄素(cirsilineol)在该色谱条件下获良好分离,分别在0 . 0 35～0 5 6 ,0 . 0 4 3～0 . 6 9,0 . 0 4 0～0. 6 5 ,0 . 0 4 0～0 6 4 μg线性关系良好(r =0 . 9992 ,0 9999,0 . 9997,0 9997) ,加样回收率分别为96. 8% ,97. 3% ,96. 8% ,98 .5 % ,重复性分别为2 . 5 % ,3 0 % ,1 5 % ,3. 2 %。结论:该方法分离度好,为花蕾期茵陈及其制剂的质量控制提供。
Objective: To develop a quantitative method for the determination of four flavonoids in buds of Herba Artemisiae Scopariae. Method: The sample was extracted by ultrasonic with ethyl acetate for 30 minutes and separated on kromasil ODS column with mobile phase of acetonitrile-tetrahydrofuran-1% acetic acid solution(2.5∶21.5∶76)and detection wavelength was 347 nm. Result: The arcapillin, cirsiliol, cirsimaritin and cirsilineol in bud extract were well separated by this method. Linearities of arcapillin, cirsiliol, cirsimaritin and cirsilineol were good(r=0.999 2,0.999 9,0.999 7,0.999 7)in ranges of 0.035-0.56,0.043-0.69,0.040-0.64 and 0.040-0.64 μg,respectively. The average recoveries were 96.8 %,97.3%,96.8%,98.5% and RSD the values of repeatability were 2.5%,3.0%,1.5%,3.2%, respectively. Conclusion: The method validation data indicated that the method is reliable and it can be used for quality control of Herba Artemisiae Scopariae and its preparations.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第8期591-594,共4页
China Journal of Chinese Materia Medica
基金
国家重点科技攻关项目 (96 90 3 0 2 0 2 )
