摘要
AIM： To study the effects of lysophosphatidic acid （LPA） on proliferation, adhesion, migration, and apoptosis in the human colon cancer cell line, SW480, and its mechanisms of action. METHODS： Methyl tetrazolium assay was used to assess cell proliferation. Flow cytometry was employed to detect cell apoptosis. Cell migration was measured by using a Boyden transweU migration chamber. Cell adhesion assay was performed in 96-well plates according to protocol. RESULTS： LPA significantly stimulated SW480 cell proliferation in a dose-dependent and timeependent manner compared with the control group （P 〈 0.05） while the mitogen-activated protein kinase （MAPK） inhibitor, PD98059, significantly blocked the LPA stimulation effect on proliferation. LPA also significantly stimulated adhesion and migration of SW480 cells in a dosedependent manner （P 〈 0.05）. Rho kinase inhibitor, Y-27632, significantly inhibited the upegulatory effect of LPA on adhesion and migration （P 〈 0.05）. LPA significantly protected cells from apoptosis induced by the chemotherapeutic drugs, cisplatin and 5-FU （P 〈 0.05）, but the phosphoinositide 3-kinase （PI3K） inhibitor, LY294002, significantly blocked the protective effect of LPA on apoptosis. CONCLUSION： LPA stimulated proliferation, adhesion,migration of 5W480 cells, and protected from apoptosis. The Ras/Raf-MAPK, G12/13-Rho-RhoA and PI3K- AKT/PKB signal pathways may be involved.
AIM:To study the effects of lysophosphatidic acid(LPA) on proliferation,adhesion,migration,and apoptosis in the human colon cancer cell line,SW480,and its mechanisms of action. METHODS:Methyl tetrazolium assay was used to assess cell proliferation.Flow cytometry was employed to detect cell apoptosis.Cell migration was measured by using a Boyden transwell migration chamber.Cell adhesion assay was performed in 96-well plates according to protocol. RESULTS:LPA significantly stimulated SW480 cell proliferation in a dose-dependent and time-dependent manner compared with the control group(P<0.05) while the mitogen-activated protein kinase(MAPK)inhibitor,PD98059,significantly blocked the LPA stimulation effect on proliferation.LPA also significantly stimulated adhesion and migration of SW480 cells in a dosedependent manner(P<0.05).Rho kinase inhibitor, Y-27632,significantly inhibited the up-regulatory effect of LPA on adhesion and migration(P<0.05).LPA significantly protected cells from apoptosis induced by the chemotherapeutic drugs,cisplatin and 5-FU(P<0.05), but the phosphoinositide 3-kinase(PI3K)inhibitor, LY294002,significantly blocked the protective effect of LPA on apoptosis. CONCLUSION:LPA stimulated proliferation,adhesion,migration of SW480 cells,and protected from apoptosis.The Ras/Raf-MAPK,G12/13-Rho-RhoA and PI3K- AKT/PKB signal pathways may be involved.
