摘要
目的探讨非小细胞肺癌(Non-Small CellLung Cancer,NSCLC)肿瘤间质淋巴管生成与临床病理因素之间的关系。方法应用抗Podoplanin单克隆抗体(Elivision法)对68例NSCLC术后石蜡切片进行染色,观察肿瘤间质和癌旁组织淋巴管密度(Lymphatic vessel density,LVD)。结果①癌旁组织中淋巴管密度平均值为7.50±0.21(3.5～11.4),肿瘤组织间质中LVD为11.91±0.60(4.5～21.5),两组相比差异有统计学意义(P<0.05);②按有无淋巴结转移分组,68例NSCLC肿瘤组织间质中淋巴结阳性患者LVD平均值为15.73±0.74,淋巴结阴性患者LVD平均值为8.31±0.32,两组相比差异有统计学意义(P<0.05);以(高分化+中分化)和(低分化+未分化)进行分组,其LVD分别为9.42±0.54和15.46±0.87,两组相比差异有统计学意义(P<0.05);以Ⅰ、Ⅱ和Ⅲa期进行分组,其LVD分别为8.21±0.32,15.19±1.04和15.20±1.01,各组内差异有统计学意义(P<0.05)。结论非小细胞肺癌组织中淋巴管密度在分期较晚的肿瘤和有淋巴结转移的肿瘤中显著增高,从而有望为NSCLC抗淋巴管生成的研究提供理论依据。
Objective To explore the relationships between tumor stroma lymphangiogenesis in non-small cell lung cancer （NSCLC） and clinicopathologic factors. Methods Podoplanin was detected in 68 paraffin sections of NSCLC by immunohistochemistry（Elivision Methods）. The Lymphatic vessel density（LVD） in the tumor stroma and adjacent tissues was observed. Results ①LVD in the adjacent tissues of NSCLC （ 7.50 ± 0.21 ） was significantly lower than that in the tumor stroma （ 11.91 ± 0.60） ,P 〈 0.05. ②LVD in the group with metastasis of lymph node was significantly higher than another group without it （ 15.73 ± 0.74 vs 8.31 ± 0.32）, P 〈 0.05, there was significant difference between the well differentiated group and the poor differentiated group （ 9.42 ± 0. 54 vs 15.46 ± 0.87 ）, P 〈 0.05, LVD in the I stage （ 8.21 ± 0.32 ） was significant lower than that in Ⅱ,Ⅲa stages（15.19 ± 1.04,15.20 ± 1.01 ） ,P 〈0.05. Conclusion Lymphangiogenesis is closely related to tumor stages and lymph node metastasis. This result provides a theoretical basis for the research of anti-lymaphangiogenesis.
出处
《中华全科医学》
2009年第11期1148-1149,F0003,共3页
Chinese Journal of General Practice
基金
安徽省教育厅自然科学研究资助项目(KJ2008B53ZC)
关键词
非小细胞肺癌
淋巴管生成
淋巴管密度
癌旁组织
Non-Small Cell Lung Cancer
Lymphangiogenesis
Lymphatic vessel density
Adjacent tissues
