摘要
目的:探讨Ki67和survivin蛋白的表达及与甲状腺癌的关系。方法:用免疫组化检测199例甲状腺石蜡标本的Ki67和survivin蛋白表达情况,其中甲状腺癌76例(乳头状癌35例、滤泡癌22例、髓样癌19例);甲状腺良性肿瘤72例;癌旁甲状腺组织41例;正常甲状腺组织10例。结果:Ki67蛋白表达在甲状腺癌组织、甲状腺良性肿瘤、癌旁甲状腺组织、正常甲状腺组织中的阳性率分别为76.3%(58/76)、11.1%(8/72)、17.1%(7/41)、10.0%(1/10);甲状腺癌组织中Ki67蛋白阳性表达率显著高于甲状腺良性肿瘤(χ2=15.486,P=0.002),明显高于癌旁甲状腺组织(χ2=12.264,P=0.012),显著高于正常甲状腺组织(χ2=19.652,P=0.000)。Survivin蛋白表达在甲状腺癌组织、癌旁甲状腺组织、癌甲状腺组织、正常甲状腺组织中的阳性率分别为89.5%(68/76)、13.9%(10/72)、28.1%(9/32)、10.0%(1/10);甲状腺癌组织中survivin蛋白表达阳性率显著高于甲状腺良性肿瘤(χ2=18.652,P=0.000),明显高于癌旁甲状腺组织(χ2=11.821,P=0.014),显著高于正常甲状腺组织(χ2=21.572,P=0.000)。有淋巴结转移的甲状腺癌组织中Ki67、survivin蛋白表达阳性率分别为86.4%(38/44)和88.9%(40/45);明显高于无淋巴结转移的癌组织中Ki67、survivin蛋白表达阳性率[62.5%(20/32)和77.4%(24/31),χ2=9.053,P=0.041;χ2=9.417,P=0.035]。76例甲状腺癌组织中Ki67、survivin蛋白的表达呈正相关(rs=0.301,P=0.015)。结论:Ki67和survivin蛋白在甲状腺癌组织中过表达与淋巴转移有关,有希望预测甲状腺癌的淋巴转移及成为临床治疗的靶点。
Objective To investigate the association of expressions of Ki-67 and survivin protein with thyroid cancer. Methods The expressions of Ki-67 and survivin protein were detected by immunohistochemistry in 199 samples of thyroid tissue （76 thyroid carcinoma, 72 benign thyroid neoplasm, 41 paracancerous tissue, and 10 normal thyroid tissue）. Results The expression rate of Ki-67 was 76.3% in thyroid carcinoma, 11.1% in benign thyroid neoplasm, 17.1% in paracancerous tissue, and 10.0% in normal tissue, showing a significant difference between thyriod carcinoma and benign thyroid neoplasm （x^2 = 15.486, P = 0.002）, or between thyriod carcinoma and paracancerous tissue （x^2 = 12.264, P = 0.012）, or between thyriod carcinoma and normal tissue （x^2 = 19.652, P = 0.000）; the expression rate of survivin protein was 89.5% in thyroid carcinoma, 13.9% in benign thyroid neoplasm, 28.1% in paracancerous tissue, and 10.0% in normal tissue, revealing a significant difference between thyriod carcinoma and benign thyroid neoplasm （x^2 = 18.652,P = 0.000）, or between thyriod carcinoma and paracancerous tissue （x^2 = 11.821, P = 0.014）, or between thyriod carcinoma and normal tissue （x^2= 21.572, P = 0.000）. The expression rate of Ki-67 and that of survirin were markedly higher in the cancer tissues with lymph node metastasis than in those without metastasis （86.4% vs. 62.5%, x^2= 9.053, P = 0.041; 88.9% vs. 77.4%, x^2 = 9.417, P = 0.035）. In all the cancer tissues, the expression of Ki-67 was positively related with that of survivin （rs = 0.301, P = 0.015）. Conclusions The overexpressions of Ki-67 and survivin protein are associated with lymph node metastasis in thyroid cancer. Ki-67 and survivin protein can be used as a promising predictor for lymphatic metastasis and become a target in the anticancer treatment.
出处
《实用医学杂志》
CAS
北大核心
2009年第19期3215-3218,共4页
The Journal of Practical Medicine
