摘要
本研究目的是观察饮食中添加钙和维生素D对高脂饮食诱发的小鼠乳腺上皮细胞增生的影响。将四周龄小鼠随机分成三组，分别给予对照AIN－76A饲料，高脂低钙低维生素D饲料（试验饲料Ⅰ）和高脂加钙加维生素D饲料（试验饲料Ⅱ）。饲养九周后终止试验，小鼠植入渗透泵灌注溴脱氧尿苷（Brdu）72小时。结果显示试验饲料Ⅰ组的小鼠乳腺终末小导管上皮细胞Brdu标记指数与对照饲料组比较明显升高（P＜0．05）。而试验饲料Ⅱ组的BrdU标记指数与对照组相似（P＞0．05）。本实验研究发现进一步证明，高脂饮食能诱发小鼠乳腺小导管上皮细胞的增生，同时也提示饮食中添加钙和维生素D有助于预防高脂饮食的这种不良作用。
The present study was designed to investigate the effects of added dietary calcium and vitamin D on the development of epithelial cell hyperproliferation induced in the mammary gland of mice by a high-fat diet. Four-week-old C57BL/6J mice were randomly divided into three groups, and given control diet (AIN-76A), high-fat diet with reduced calcium and vitamin D (Experimental diet i) and high-fat diet with addition of calcium and vitamin D (Experimental diet I ), respectively. Nine weeks after the dietary intervention, the animals were implanted with osmotic pumps for 72 hours to infuse bromodeoxyuridine (BrdU). Findings revealed that mice on Experimental diet I had significantly increased (P M 0. 05) BrdU--labeling indices of the terminal ducts of the mammary gland, compared to control diet group i however, BrdU--labeling indices of the terminal ducts were similar (P > 0. 05) in the control diet and Experimental diet Ⅱgroups. The present study further demonstrates that a high--fat diet induces hyperproliferation of epithelial cells in the terminal ducts, and also suggests that this effect of the high-fat diet can be prevented by increasing dietary calcium and vitamin D.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1998年第6期451-454,共4页
Cancer Research on Prevention and Treatment
关键词
乳腺上皮增生
钙
维生素D
乳腺部
预防
mammary gland
epithelial hyperproliferation
calcium
vitamin D
