摘要
亲和超滤HPLC-MS法是将超滤技术与HPLC-MS联用,主要用于从组合化学库和天然产物粗提物中筛选与可溶性靶点相结合的小分子活性物质,在药物代谢和描述受体-配体结合特征两方面也有所应用。用该方法筛选和发现药物被认为是传统药物发现方法的一种补充。本文综述了亲和超滤HPLC-MS法的原理及其在药物研究中的应用。
Affinity selection-ultrafiltration/HPLC-MS is the combination of the ultrafiltration and HPLC-MS,mainly used for screening small active molecular substances from combinatorial libraries and natural product extracts,which can bind to solution-phase targets.Besides,it can be used in metabolic screening and characterization of ligand-receptor binding.It is a complement to the traditional methods of screening and identifying drugs.This review describes its principle and application in drug study.
出处
《药学学报》
CAS
CSCD
北大核心
2009年第10期1084-1088,共5页
Acta Pharmaceutica Sinica
