摘要
通过白茶和智仁早茶在铝氟交互处理下添加外源磷的水培试验,研究了不同浓度磷(0,0.2,1.0 mmol/L)对茶树主要化学成分及抗氧化酶的影响。结果表明:(1)当施入0.2 mmol/L磷时,茶叶中的蛋白质、叶绿素和茶多酚含量及SOD、POD和CAT活性均比无磷组高,且在5.0 mmol/L铝和1.0 mmol/L氟处理下,均达到最高值(叶绿素除外),表明适量外源磷的施加对茶树的生理生化特性起促进作用,并能有效缓解铝和氟毒所致的氧化损伤,从而抑制了脂质过氧化作用;(2)随着磷浓度(1.0mmol/L)增加,茶叶中的各指标值均比无磷组和对照低,出现最低值,表明磷对铝氟胁迫下茶树的缓解效应有限,超过一定的浓度,则表现出明显的抑制作用;(3)在外源磷的施加下,白茶的可溶性蛋白、茶多酚和叶绿素含量及SOD、POD和CAT活性均比智仁早茶的变化幅度大。因此,外源磷对茶树幼苗生理生化作用具有一定的调控效应。
we studied the physiological and biochemical characteristics of tea plants leaves were affected by different concentrations of phosphate(P)(consisting of 0,0.2 and 1.0 mmol/L) through hydroponic cultivation experiments of Camellia sinensis L(baicha and zhirenzaocha) under aluminum(Al) and fluorin(F) interactions.The results follow showed:(1) Compared with the four non-P treatments,an obvious upward tendency of soluble protein,chlorophyll and polyphenols contents and superoxidedismutase(SOD),peroxidase(POD) and catalase(CAT) activity was observed in the treatment of 0.2 mmol/L P under single Al or F.Meanwhile these indexes reached maximum values except the content of chlorophyll under 5.0 mmol/L Al and 1.0 mmol/L F interactions in zhirenzaocha.This suggests proper concentration of P can weaken oxidative injury-caused by Al and/or F and inhibit lipid peroxides by increasing antioxidative enzyme acitivities.(2) With the increasing concentrations of P,the soluble protein,chlorophyll content,polyphenols contents and superoxidative dismutase(SOD),peroxidase(POD),catalase(CAT) activities were all fell to the minimum level,which were all below non-P and the control.The fact that the treatment of 1.0 mmol/L P can not alleviated the toxonosis-caused by Al and F interactions implies detoxication depends on concentration of P and beyond the concentration range,it restrains detoxication.(3) The sensibilities between baicha and zhirenzaocha according to the P stress were differed and order was sorted as follows:zhirenzaocha〉 baicha.Therefore,the concentration of P plays a high impact on physiological and biochemical characteristics of young tea plants and detoxication of P is associated with variety under Al and F interactions.
出处
《生态学报》
CAS
CSCD
北大核心
2009年第10期5434-5441,共8页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目(40573052)
浙江省自然科学基金资助项目(305151
304135)
关键词
磷
铝-氟交互
茶
生理生化特性
phosphate
physiological and biochemical characteristic
tea plants
Al and F interactions