摘要
目的观察脑心通对氧化低密度脂蛋白(ox-LDL)作用下的人THP-1细胞活力及炎症反应的影响。方法将THP-1源性巨噬细胞分为7个组,即对照组(不加任何干预)、ox-LDL组(50mg/L ox-LDL干预细胞24h)、脑心通1组、脑心通2组、脑心通3组、脑心通4组(分别以0.125g/L、0.25g/L、0.5g/L、1.0g/L脑心通和50mg/L ox-LDL与细胞共孵育24h)、阿托伐他汀组(1μmol/L阿托伐他汀和50mg/L ox-LDL与细胞共孵育24h)。噻唑蓝(MTT)比色法检测THP-1细胞活力,酶联免疫吸附法(ELISA)检测细胞上清液中白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量。结果脑心通有轻度保护ox-LDL作用下巨噬细胞活力的作用,并抑制其引起的细胞炎症反应,上述作用均呈浓度依赖性,1g/L脑心通作用最为明显。结论脑心通呈浓度依赖性抑制ox-LDL引起的细胞损伤及炎症反应。
Objective To investigate the effect of Naoxintong Capsule （Chinese prepared medicine for atherosclerosis） on cell vitality and inflammatory reaction of THP-1 macrophages induced by oxidized low density lipoprotein （ox-LDL）. Methods The THP-1 original macrophages were divided into seven groups： control group, ox-LDL group （50mg/L ox-LDL intervention for 24h）, Naoxintong group I , II , III , IV （0. 125g/L, 0. 25g/L, 0. 5 g/L, 1.0 g/L Naoxintong and 50mg/L ox-LDL incubated with macrophages for 24h）, atorvastatin group （atorvastatin 1μmol/L and 50mg/L ox-LDL incubated with macrophages for 24h）. THP-1 macrophages vitality was tested with MTT colorimetric method and the contents of IL-6 and TNF-α were detected by enzyme linked immunosorbent assay respectively. Results Naoxintong has slight cytoprotection effect and inhibits inflammatory reaction of THP-1 macrophages induced by ox-LDL in a mode of dose-dependence, and the most obvious effect was in a dose of 1.0g/L. Conclusion Naoxintong slightly inhibits the cytotoxicity and inflammatory reaction of THP-1 macrophages induced by ox-LDL in a dose-dependent effect.
出处
《中医杂志》
CSCD
北大核心
2009年第10期932-935,共4页
Journal of Traditional Chinese Medicine
关键词
脑心通
THP-1巨噬细胞
细胞活力
白细胞介素6
肿瘤坏死因子α
氧化低密度脂蛋白
Naoxintong（Chinese prepared medicine for atherosclerosis）
THP-1 macrophages
Cell vitality
Tumor necrosis factor-α
Interleukin-6
Oxidized low density lipoprotein
