摘要
采用浸渗法制备萝卜种子抗真菌蛋白Rs－AFPs粗提液进行抑菌试验，结果表明：在萝卜种子发育过程中，Rs－AFPs是在一定时期（约开花后45d）开始合成并逐步积累的，因此充分成熟的萝卜种子Rs－AFPs粗提液具有较强的抑菌活性。Rs－AFPs的耐酸性、耐碱性、热稳定性及对不同病原真菌的抗性研究结果表明，在pH为4和10时．Rs－AFPs能保持90％的活性；在80℃水浴60min其活性仍为100％，在100℃水浴30min和45min，分别具有80％和50％的活性；在4℃下存放5、20、35、45d，Rs－AFPs的活性分别保持100％、90％、80％和70％。Rs－AFPs对植物丝状病原真菌具有广谱抗性，如对供试病原菌镰刀菌、疫霉菌、芭蕉炭疽菌和稻瘟菌均表现出颇强的抑菌活性，Rs－AFPs无抗细菌活性。
The biological properties of Raphanus sativus-antifungal proteins (Rs - AFPs )was studied by using radish seed inhibition solution. Rs -AFPs extracts were obtained from radish seeds at various development stages. Results demonstrated that Rs -AFPs began to be synthesized at a certain seed development stage (at some 45 days after anthesis ) and accumulated gradually and that Rs - AFPs extracts from fully mature radish seeds exhibited higher antifungal activity than those from immature ones. The stability of antifungal activity of Rs-AFPs under acid and alkaline and heat conditions was determined. Results disclosed that Rs - AFPs maintained 90% of their activity under pH4 and pH10. The Rs -AFPs extracts kept at 80℃ for 60 min were found to have 100% activity, and at 100℃ for 30 min and 45 min 80% and 50% respectively. The extracts stored at 4℃ for 5,20,35 and 45 days still had an activity of 100%,90%,80% and 70% respectively.Rs -AFPs exhibited potent antifungal activity against a broad spectrum of filamentous pathogenic fungi of plants,such as Colletotrichum musae,Piricularia oryzae,Fusarium oxysorum and Phytophthora citriophthora. But Rs -AFPs was not resistant to bacteria.
出处
《热带作物学报》
CSCD
1998年第2期36-42,共7页
Chinese Journal of Tropical Crops
关键词
抗真菌蛋白
病原真菌
耐酸碱性
热稳定性
萝卜
antifungal protein
pathogenic fungus
acid and alkaline resistance
heat stability
