摘要
目的:观察PPARγ激动剂吡格列酮和拮抗剂GW9662对巨噬细胞脂质蓄积和MMP9 mRNA表达的影响。方法:提取昆明小鼠腹腔巨噬细胞,以oxLDL作为泡沫细胞诱导剂,分组培养24h和72h。油红O染色观察细胞脂质蓄积情况,聚合酶链反应检测细胞MMP9 mRNA表达的变化。结果:在24h点,GW9662能抑制oxLDL诱导的巨噬细胞脂质蓄积;另一方面,在24h和72h两个时间点,吡格列酮能抑制oxLDL诱导的巨噬细胞MMP9 mRNA表达(分别为0.017和0.038)。结论:GW9662可能对动脉粥样硬化斑块病变的巨噬细胞泡沫化过程起拮抗作用,而吡格列酮在维持动脉粥样硬化斑块稳定性方面有潜在作用。
OBJFKTIVE To investigate the effects of PPARγ agonist pioglitazone and PPARγantagonist GW9662 on the lipid accumulation and the MMP9 mRNA expression in peritoneal macrophages. METHODS Peritoneal macrophages were collected from Kunming mice. oxLDL was administered as foam cell revulsant. Then the cells were incubated with different agents and cultured for 24 h and 72 h. The cellular lipid accumulation was evaluated by oil red O staining. The expression of MMP9 mRNA was analyzed by reverse transcription polymerase chain reaction. RESULTS At the end of 24 h GW9662 alleviated lipid accumulation induced by oxLDL, oxLDL significantly augmented macrophage MMP9 mRNA expression. Addition of pioglitazone diminished this effect of oxl.DL at both 24 h and 72 h （the values were 0. 017 and 0. 038 respectively）. CONCLUSION GW9662 may inhibit the foam-cell formations in atherosclerosis and pioglitazone can enhance the stability of atherosclerotic plaque.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2009年第19期1642-1644,共3页
Chinese Journal of Hospital Pharmacy
