摘要
探讨了提取液比例、pH值、提取缓冲液、浸提时间、PVP用量、提取介质、SDS等因素对胡萝卜种子蛋白质提取的影响。结果表明:蛋白质的提取受料液比、pH值、浸提时间、缓冲液种类、提取介质的影响较大。初步确定,在4℃条件下,胡萝卜种子蛋白提取的较佳条件为:料液比(W∶V)1∶10,pH值8.0的50mmol.L-1Tris-HCL缓冲液,浸提时间1 h。
Factors including the ratio of sample to solution(W∶V),pH value,buffers,time,amount of PVP,media and SDS that might affect the extraction of proteins from carrot seeds were studied.The results showed that the extraction was significantly affected by the ratio of sample to solution,pH,buffers,time and medium composition.Based on the preliminary results,protein extraction from carrot seeds for 1.0 h at 4℃ in 50 mmol·L^-1 Tris-HCl buffer with a 1∶10 ratio of sample to solution and pH 8.0 produced the best result.
出处
《福建农业学报》
CAS
2009年第4期338-340,共3页
Fujian Journal of Agricultural Sciences
基金
厦门市科技项目(3502Z20062004)
关键词
胡萝卜
种子蛋白
料液比
提取缓冲液
浸提时间
提取介质
carrot
seeds protein
ratio of sample to solution
extraction buffer
extraction time
extraction mediums