摘要
In this study,PCR product amplified with a RAPD marker A10555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum（Dendranthema grandiflorum） was recovered,and subsequently subcloned and sequenced. A pair of 18-base specific primers was designed based on the obtained sequence.The validity of this SCAR marker was verified by screening parents and 152 individuals of the F1 progeny,and the consistence in the locus of the special band and the number of recombinants was observed between SCAR analysis and RAPD analysis. The size of this SCAR marker was 555 bp,which was designated as SCA10555. The results demonstrated that the RAPD marker was converted to SCAR marker successfully. This study provides bases for new cultivars developing,molecular markers assisted breeding and creeping habit related genes cloning of the ground-cover chrysanthemum.
In this study,PCR product amplified with a RAPD marker A10_555 which is linked to gene controlling creeping habit of ground-cover chrysanthemum（Dendranthema grandiflorum） was recovered,and subsequently subcloned and sequenced. A pair of 18-base specific primers was designed based on the obtained sequence.The validity of this SCAR marker was verified by screening parents and 152 individuals of the F1 progeny,and the consistence in the locus of the special band and the number of recombinants was observed between SCAR analysis and RAPD analysis. The size of this SCAR marker was 555 bp, which was designated as SCA10555 . The results demonstrated that the RAPD marker was converted to SCAR marker successfully. This study provides bases for new cultivars developing, molecular markers assisted breeding and creeping habit related genes cloning of the ground-cover chrysanthemum.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2009年第9期147-150,共4页
Scientia Silvae Sinicae
基金
教育部新世纪优秀人才支持计划(NCET-06-0489)
国家科技支撑计划(2006BAD01A18)
江苏省高技术研究项目(BG2007315)
关键词
地被菊
株型匍匐性
SCAR标记
ground-cover chrysanthemum（Dendranthema grandiflorum）
creeping
SCAR marker
