摘要
以萝卜３个不育系Ａ１、Ａ５、Ａ６与２个自交系５４、５５号配组的６个Ｆ１代及亲本为材料，分别在营养生长期取根、叶，生殖生长期取茎生叶、花蕾，对酯酶（ＥＳＴ）、α－淀粉酶（α－Ａｍｙｌａｓｅ）、细胞色素氧化酶（ＣＯＤ）、过氧化物酶（ＰＯＤ）进行比较研究。将各时期、各部位的同工酶酶带按Ｒｆ值大小排列，酯酶共有２１条带，分为快、中、慢３个区；随着植株生长，α－淀粉酶的３条酶带Ｒｆ值有增加趋势；ＣＯＤ共有１１条带，其中有Ｒｆ０．１３和０．１７两条保守酶带；ＰＯＤ共有８条带，有Ｒｆ０．１７一条保守酶带。Ｆ１代与双亲各种酶谱相比大多表现为双亲互补型，没有发现杂种酶带。
Four isozymes including esterase (EST), α amylase, cytochrome (COD) and peroxidase (POD) of roots and leaves at vegetative stage, and of stem leaves and flower buds at reproductive stage were compared in six F 1 and their parent lines. After adding up the bands, which lined in Rf order, of all developmental stages and tissues, there were 24 bands divided into fast, middle and slow parts in EST. Rf of three bands in α amylase were ascended during plant growth; there were 11 bands in COD, two of which (Rf=0.13, 0.17) were constant; and there were 8 bands in POD, one of which (Rf=0.17) was constant. Zymograms of most F 1 hybrids are complementary pattern of parents. No hybrid bands was found.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1998年第4期31-35,共5页
Journal of Nanjing Agricultural University
关键词
萝卜
同工酶
电泳
遗传
radish
isozyme
electrophoresis
