摘要
目的探讨PINCH(particulary interesting new cysteine-histidine rich protein,PINCH)蛋白在胃腺癌和胃黏膜非典型增生、正常胃黏膜中的表达情况,分析PINCH蛋白表达与患者年龄、性别、肿瘤大小、部位以及淋巴结转移的关系。方法采用免疫组织化学SP法,检测30例正常胃黏膜、36例非典型增生胃黏膜及70例胃腺癌石蜡包埋标本中PINCH蛋白的表达,并分析其临床意义。结果 PINCH蛋白在胃腺癌组织相关间质中呈异质型表达,其阳性率为74.28%(52/70),明显高于正常胃黏膜组织,差异有统计学意义(P<0.05);而PINCH蛋白胃黏膜非典型增生中的表达阳性率为38%(14/36),与正常胃黏膜相比,差异无统计学意义(P>0.05);而且PINCH蛋白在肿瘤浸润的边缘阳性更为明显;在胃腺癌中,淋巴结转移组阳性表达率为77.78%(35/45),淋巴结无转移组表达率为48.00%(12/25),二者比较,差异有统计学意义(P<0.05);PINCH蛋白的表达与患者性别、年龄、肿瘤大小、部位无相关性(P>0.05)。结论 PINCH蛋白在胃腺癌的发生、发展中可能起着重要作用,并与肿瘤的浸润和转移有关。
Objective To investigate the expression of particulary interesting new cysteinehistidine rich protein （PINCH） protein in gastric adenocarcinoma and atypital hyperplasia and normal gastric mucosa,and to analyze the correlation Of PINCH expression and clinicopathologic features such as patient＇s gender, age, tumor location, tumor size, and lymph node metastasis. Methods SP immunohistochemical method was used to detect the expression of PINCH in paraffin block from 30 cases of normal gastric mucosa tissue,36 cases of atypical hyperplasia and 70 cases of gastric adenocarcinoma. The relationship between the expression of PINCH and the patients clinicopathologic features was analyzed. Results The expression of PINCH protein in tumorassociated stromal cells of gastric adenocarcinoma was heterogeneous and the positive rate was 74.28%（52/70） ,which was higher than that of normal gastric mucosa （ P 〈0.05）. The positive rate was 38% （14/36） in atypical hyperplasia and there was no significant difference between normal mucosa group and atypical hyperplasia group （ P 〉 0.05）. The expression of PINCH at invasive edges was obviously higher. The positive rate of the lymph node metastasis group （77. 78%, 35/45） was significantly higher than that of non-lymph node metastasis group （48.00%,12/25） （P〈0.05）. PINCH expression was not obviously correlated with patients
出处
《河北医科大学学报》
CAS
2009年第5期485-488,共4页
Journal of Hebei Medical University
基金
河北省2007年科学技术与发展指导计划资助项目(072761950)
关键词
蛋白质类
胃肿瘤
免疫组织化学
proteins
stomach neoplasms
immunohistochemistry
