摘要
目的采用HPLC测定合成益母草碱的含量。方法用C18色谱柱,流动相为0.025 mol·L^-1磷酸二氢钾溶液（磷酸调pH4）-甲醇（17：83）;流速0.8 ml·min^-1;检测波长277 nm,柱温为30℃。结果益母草碱进样量6.09～60.09μg·ml^-1与峰面积的线性关系良好（r=0.9998）,平均回收率为99.78%（RSD=0.55%,n=6）。结论所建方法灵敏、准确、重复性好,操作简便,可有效地测定益母草碱的含量。
OBJECTIVE To establish an HPLC method for the determination of the content of Leonurine. METHODS A C18 column was used. A flow rate of mobile phase was at 0.8 ml· min^-1. The mobile phase was a mixture of MeOH and KH2 PO4 solution （0. 025 mol·l^-1, adjust with phosphoric acid to pH4,83 ： 17 ）. An isoeratic elution mode was carried out with the column temperature at 30℃. The UV detection wavelength was at 277 nm. RESULTS The calibration curve of rivastigmine showed a good linear regression within the range of 6.09 -60.09 μg· ml^-1（ r = 0. 9998 ,n = 6）. The average recovery was 99.78% with RSD of 0.55% （n = 6）. CONCLUSION The HPLC method is accurate and sensitive for the determination of Leonurine.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2009年第5期546-547,共2页
West China Journal of Pharmaceutical Sciences
