摘要
[目的]优化魔芋组织培养技术,解决种芋携带病原物与病原物累积,使病害逐年加重,造成损失的问题。筛选出一个适宜的魔芋茎尖试管苗培养基,并优化其激素组成和配比。[方法]以魔芋组培苗丛生芽茎尖为外植体,接种到7个不同配方的培养基上培养,选出适宜的基础培养基;再以之为基础,选择6-BA、NAA、GA3进行3因素3水平正交试验,优化其配比。[结果]MS+2.0mg6-BA+0.2mgNAA+0.2mgGA3+30mg蔗糖+6mg琼脂(pH6.0)是魔芋茎尖试管苗适宜的基础培养基。优化试验表明:2.0mg/L6-BA+0.1mg/LNAA+0.2mg/LGA3的激素组合表现最好。[结论]因子分析认为各激素的最佳组合应为2.0mg/L6-BA+0.1mg/LNAA+0.3mg/LGA。
[Objective ] Optimizing tissue culture technique of Amorphophallus, and resolved the loss problems caused by the seed-tuber carrying pathogen and pathogen accumulated which made disease seriously year by year. Screening a suitable medium of test-tube shoots from stem Apexes of Amorphophallus, and optimizing its hormone composition and proportion. [ Method ] To screen the optimal basic medium, stem apexes from shoot duster were inoculated in seven different mediums. Base on these experiments, the combinations of hormones including 6-BA, NAA and GA3 were optimized by orthogonal experimentation. [ Result] The optimal basic medium of test-tube shoots from stem apexes was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.2 mg/L ＋ GA3 0.2mg/L ＋ sucrose 30 g/L ＋ agar 6 g/L （ pH 6.0）. Then, the results showed that the best combination of hormones was 6-BA 2.0 mg/L ＋ NAA 0.1 mg/L ＋ GA3 0. 2 mg/L by orthogonal experimentation, but the factor analysis showed that the best one was 6-BA 2.0 mg/L ＋ NAA 0.1 mg/L ＋ GA30. 3 mg/L .
出处
《安徽农业科学》
CAS
北大核心
2009年第30期14627-14628,共2页
Journal of Anhui Agricultural Sciences
基金
国家支撑计划魔芋试管苗工厂化生产和标准化繁供体系建设项目子课题(2007BAD7304-1)
湖北省农业科技创新中心魔芋育种团队资助
关键词
魔芋
茎尖
培养基
筛选
AmorphophaUu
Stem apex
Medium
Screen
