摘要
对分离自酸马奶酒中的两株产抑菌物质的乳酸菌BY-3-2、SQ-3-2进行分离鉴定及系统发育分析,以更准确地判定其属种。对菌株进行生理、生化特性鉴定及其16SrDNA序列分析。结果表明:菌株BY-3-2、SQ-3-2的生理、生化特性符合肠球菌属特征,其中BY-3-2菌株符合E.faecium的特征;SQ-3-2菌株符合E.durans的特征。通过16SrDNA序列进行系统发育分析,结果与生理生化鉴定相一致。
In order to identify genus and species of two stains of lactic acid bacteria with antibacterial functions, BY-3-2 and SQ-3-2 were separated from Koumiss. Their physiological and biochemical characteristics and 16S rDNA sequence were analyzed. Results indicated that physiological and biochemical characteristics of BY-3-2 and SQ-3-2 were the same as those of Enterococcus. Moreover, strain BY-3-2 had the characteristics of E.faecium, and SQ-3-2 had the characteristics orE. durans, which was also supported by 16S rDNA sequence analysis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第19期232-235,共4页
Food Science
基金
国家自然科学基金资助项目(29966003)
关键词
乳酸菌
分离鉴定
16S-rDNA序列分析
lactic acid bacteria
isolation and identification
16S rDNA sequence analysis
