摘要
目的:观察大剂量甲基强的松龙(MP)治疗对大鼠急性脊髓损伤(ASCI)后神经细胞凋亡及凋亡基因Bcl2的影响。方法:选取48只雌性SD大鼠随机等分为2组,对照组与治疗组,按Nystr觟m法制备大鼠急性脊髓损伤模型。治疗组伤后30min经腹膜腔注入MP30mg/kg,以后每小时腹膜腔注入MP5.4mg/kg,维持24h;对照组应用生理盐水替代MP,处理方法同治疗组。两组分别于伤后4、8h及1、3、7、14d灌注固定后取材。免疫组织化学检测损伤段脊髓内Bcl2蛋白表达,TUNEL检测细胞凋亡,染色结果应用图像分析仪进行半定量分析。结果:大鼠ASCI后4h即可见脊髓内TUNEL阳性细胞,8h表达达高峰,此后表达量逐渐下降,14d时仍可见少量阳性细胞。凋亡相关蛋白Bcl2在伤后4h即可见表达,伤后1d达高峰,伤后14d仍有表达。与对照组相比,治疗组伤后8h、1d和3d时凋亡细胞数减少有统计学意义,伤后8h和1dBcl2蛋白表达增高有统计学意义。结论:大剂量甲基强的松龙治疗可抑制大鼠ASCI后神经细胞凋亡,并增加凋亡相关蛋白Bcl2的表达。
Objective：To investigate the effect of high dose methylprednisolone （MP） on cell apoptosis and Bcl-2 expression after acute spinal cord injury （ASCI）. Methods：The 48 female rats were randomly divided into two groups：control group and experimental group. The spinal cord of rats were wounded at the level of T9.10 in moderate degree for each group. Thirty minutes after ASCI,the subjects in experimental group received MP injection through intraperitoneal with dosage of 30 mg/kg. Then a dosage of 5.4 mg/kg of MP was given through intraperitoneal injection every hour in 24 hours. The subjects in the control group received Normal Saline instead of MP with the same method. The impaired spinal cords were harvested on 4 h, 8 h, 1 d,3 d,7 d and 14 d after injury,and at each time point 4 rats were sacrificed in each group. The terminal deoxynucleotidal transferase-mediated DUTP-biotin nick end labeling （TUNEL） and immunohistochemical measurement were used to observe neural apoptosis and Bcl-2 expression. Results ：Apoptosis cells were noted primarily at 4 h after injury,with a maximum presence in lesion center at 8 h. The apoptotic index of MP-treated group had distinctly less than that of control group in 8 h, 1 d and 3 d after trauma. The Bcl-2 increased in the experimental group. Conclusion ：The administration of the high dose methylprednisolone （MP） can inhibite the apoptosis after acute spinal cord injury in rats and increase the expression of Bcl-2.
出处
《中国骨伤》
CAS
2009年第9期692-694,共3页
China Journal of Orthopaedics and Traumatology
基金
国家自然科学基金资助项目(编号:30500509)
河北省自然科学基金资助项目(编号:C2006000840)
