摘要
为了解能够表征盐胁迫下小麦叶片细胞膜变化的一些电参数特性,应用电阻抗图谱方法研究小麦叶片对不同盐浓度溶液胁迫的反应,拟合叶片的胞外电阻、胞内电阻、弛豫时间及弛豫时间分布系数,分析盐浓度和胁迫时间对各个电阻抗图谱参数的影响以及这些参数彼此之间的相关性.结果表明:随着盐浓度和胁迫时间的增加,胞外电阻和弛豫时间表现为先增加后减小或者逐渐减小的变化趋势;在胁迫时间小于12 h或者盐浓度小于100 mmol·L-1时叶片的胞外电阻变化较剧烈,胞内电阻和弛豫时间分布系数先减小后增大;4个电阻抗图谱参数在时间上的差异极显著,在浓度上的差异只有胞内电阻不显著,其他参数均为极显著;胞外电阻和弛豫时间呈极显著正相关,与弛豫时间分布系数呈极显著负相关,弛豫时间与弛豫时间分布系数呈显著负相关.
To find out the traits of electrical parameter in cell membrane of wheat leaves under salt stress, electrical impedance spectroscopy （EIS） was applied to follow the reaction of impedance parameters at different NaCl concentrations. Four EIS parameters of extracellular resistance, intracellular resistance, relaxation time and distribution coefficient of relaxation time in wheat leaves were fitted. The effect of NaCl concentration and stress time on EIS parameters and correlation among these parameters were analyzed. The results show that extracellular resistance and relaxation time increased firstly and then decreased, or decreased gradually with increasing stress time and NaCl concentration. Extracellular resistance changed rapidly when stress time was less than 12 h or NaCl concentration less than 100 mmol. L-1 , whereas intracellular resistance and distribution coefficient of relaxation time then increased. concentration e relaxation time Vo xcep and correlation between ur EIS parameters differed significantly in stress time and the t intracellular resistance. Extracellular resistance was correlated negatively with distribution coefficient of relaxation time. There relaxation time and distribution coefficient of relaxation time. first decreased, same in NaCI positively with was a negative
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2009年第5期564-568,共5页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家科技部"西部开发"重大资助项目(2004BA901A13)
关键词
盐胁迫
小麦叶片
电阻抗图谱
salt stress
wheat leaves
electrical impedance spectroscopy
