摘要
目的:建立同时测定升阳散火汤中葛根素、阿魏酸、异阿魏酸、蛇床子素和异欧前胡素5种活性成分含量的RP-HPLC法。方法:采用Diamonsil C18(200mm×4.6mm,5μm)色谱柱,流动相为甲醇-4%醋酸水溶液,梯度洗脱;流速1.0mL.min-1;紫外检测波长310nm;柱温35℃。结果:葛根素、阿魏酸、异阿魏酸、蛇床子素和异欧前胡素线性范围分别为15.6～156μg·mL-1(r=0.9995),0.660～6.60μg·mL-1(r=0.9997),8.96～89.6μg·mL-1(r=0.9995),6.30～63.0μg·mL-1(r=0.9993),1.32～13.2μg·mL-1(r=0.9996);方法平均回收率(n=9)分别为99.7%,100.6%,100.2%,100.6%,99.8%。结论:本法简便、准确,重复性好,可为升阳散火汤质量评价提供依据。
Objective ： To develop an RP - HPLC method for the simultaneous determination of 5 components （ puerarin,ferulic acid, isoferulic acid, osthol and isoimperatorin）in Shengyang Sanhuo powdered extract. Methods：An RP- HPLC method was applied with Diamonsil C ls column （200 mmx 4.6 ram,5 μm）by gradient elution using methanol -4% acetic acid water solution as the mobile phase at a flow rate of 1.0 mL · min-1. The UV detection wavelength was set at 310 nm and the column temperature was 35 ℃. Results：The linear ranges of puerarin, ferulic acid, isoferulic acid, osthol and isoimperatorin were 15.6 - 156 μg · mL - 1 （ r = 0. 9995 ） ,0. 660 - 6.60μg · mL - 1 （r=0.9997）,8.96-89.6 μg · mL - 1（r=0.9995）,6.30-63.0μg · mL - 1（r=0.9993）,1.32-13.2 μg · mL - 1（r= 0.9996 ）, respectively ; The average recoveries （ n = 9 ） of puerarin, ferulic acid, isoferulic acid, osthol and isoimperatorin were 99.7%, 100.6% , 100.2% , 100.6% ,99.8%, respectively. Conclusion：This method is suit- able to control the quality of Shengyang Sanhuo powdered extract, which provides the advantages of simplicity, accu- racy and reproduction.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第9期1415-1419,共5页
Chinese Journal of Pharmaceutical Analysis
