摘要
采用藻类生物测试标准方法研究了不同NO2--N初始浓度条件下铜绿微囊藻(Microcystis aeruginosa)的生长及生理变化.结果表明,相对BG11(NO2--N初始浓度为0)培养基,低初始NO2--N浓度(1,10mg/L)条件下铜绿微囊藻生长良好;高NO2--N初始浓度(20,30,40mg/L)条件下藻生长缓慢甚至停滞.NO2--N初始浓度由10mg/L到30mg/L,亚硝酸还原酶(NiR)活性和过氧化氢酶(CAT)活性呈增加趋势;在NO2--N初始浓度分别为20～30mg/L和20～40mg/L时,叶绿素a(Chl-a)含量和丙二醛(MDA)含量随NO2--N浓度增大而逐渐升高;在初始NO2--N浓度0,1,10,20,30mg/L条件下硝酸还原酶(NR)没有明显变化.这表明,铜绿微囊藻在NO2--N初始浓度10,20,30mg/L条件下能维持一定的生长,主要由于藻叶绿素含量的增加,NiR活性和防止细胞过氧化的酶CAT活性的提高所致.
The growth and physiological change ofMicrocystis aeruginosa were studied under different initial NO2-N concentration, adopting the standard method of algal bioassay. Compared with BG11 （without NO2-N）, M. aeruginosa grew well under low initial NO2^- -N concentration （1 and 10mg/L）, however, the growth slowed down and even ceased under high initial NO2^--N concentration （from 20 to 40mg/L）. The nitrite reductase （NiR） activity and catalase （CAT） activity tended to increase from initial NO2 -N concentration of 10 to 30mg/L. The content of chlorophyll a （Chl-a） and malondiadehyde （MDA） also showed an increase with initial NO2 -N concentration increasing in the range of 20 to 30mg/L and 20 to 40mg/L respectively. The nitrate reductase （NR） activity was constant under initial NO2-N concentration （from 0 to 30mg/L）. These observed phenomena indicated M. aeruginosa could maintain definite growth in initial NO2 -N concentration rage of 10 to 30mg/L, due to the increase of Chl-a content and the enhancing of enzymic activity of NiR and CAT.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2009年第9期972-976,共5页
China Environmental Science
基金
天津大学-南开大学合作基金(AJ0013)
