摘要
建立了测定安痛定注射液中氨基比林和安替比林含量的高效液相色谱法。采用W aters公司Symmetry C18（4.6 mm×250 mm,5μm）色谱柱;以甲醇-水（40∶60）为流动相;检测波长为260 nm;流速为1.0 mL/m in;柱温25℃。结果表明：氨基比林在20-200μg/mL的浓度范围内呈线性关系,r=0.999 8,平均回收率为100.4%;安替比林在10-60μg/mL的浓度范围内呈线性关系,r=0.999 7,平均回收率为100.2%。该法简便、准确可靠,可应用于安痛定注射液的质量控制。
A method for simultaneous determination of aminophenazone and phenazone in antondine injection by HPLC was established.The experiment used Waters Corporation Symmetry C18（4.6 mm× 250 mm,5 μm） column,methanol-water（40∶60） as mobile phase,the detection wavelength was 260 nm with flow rate of 1.0 mL·min-1 and column temperature of 25 ℃.Results showed that linear range of aminophenazone was 20 - 200 μg·mL-1 with r of 0.999 8,average recovery was 100.4%.Linar range of phenazone was 10 - 60 μg·mL-1 with r of 0.9997 ,the average recovery was 100.2%. This method was simple,accurate and reliable. It could be used in drug quality control.
出处
《中国兽药杂志》
2009年第9期5-7,共3页
Chinese Journal of Veterinary Drug
