摘要
目的:研究特异性鸡蛋黄抗体(IgY)对人工釉质龋形成的影响。方法:采用人工菌斑模型研究IgY对早期釉质龋形成的影响。56块釉质块经唾液包被后置6种与菌斑形成有关的混合细菌液中培养。实验组釉质块每两天置IgY液中浸泡处理,在实验第7、14、21、28、35天分别取出对照、实验组釉质块,磨片后偏光显微镜下观察,并采用显微X线照相系统投照,TMR软件分析早期釉质脱矿量、龋损的深度。结果:对照组较实验组更早出现釉质龋,实验组釉质脱矿量为1275.2 vol%μm,显著低于对照组2836.5 vol%μm。结论:特异性IgY在体外能有效减缓早期釉质龋的发生。
AIM： To study the effect of egg - yolk IgG （IgY） on early enamel caries in vitro. METHODS ： The models of artificial plaque was used in this study. In brief, fifty - six enamel blocks were coated with saliva and then were cultured with 6 different kinds of bacteria isolated from human dental plaque. The experimental blocks were treated with IgY every 2 days. On day 7, 14, 21,28 and 35 the enamel blocks were cut into slides and observed under polarized - light microscope and X - ray generator. The quantities of enamel demineralization were analyzed using mineral analysis software. RESULTS： The enamel demineralization was observed earlier in the control group than in the experimental group. The depth of subsurface demineralization was significantly reduced from 2836.5 vol%um to 1275.2 vol%um （P = 0. 002） when enamel was treated by IgY. CONCLUSION： Specific IgY can prevent the development of enamel caries in vitro.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2009年第9期503-505,540,共4页
Chinese Journal of Conservative Dentistry
基金
湖北省科技攻关项目(982P1602)
