摘要
创造限制酶切位点PCR法是应用引物错配技术结合单核苷酸多态性(SNP)而配合成一个酶切位点,使SNP可用于PCR-RFLP分析的一种方法。应用CRS-PCR技术检测了大口黑鲈(Micropterus salmoides)IGF-I基因内含子1上SNPG 208A的多态性,并详述了CRS-PCR错配引物的设计方法,CRS-PCR引物设计和应用的注意事项,以促进CRS-PCR单核苷酸多态检测方法在水产动物研究领域的应用。
Created restriction site PCR （CRS-PCR） uses mismatches in one of the two PCR primers flanking the single nucleotide polymorphism to create or remove a restriction endonuclease recognition site in order to identify SNP genotypes. In this study, the genotype of SNP G208A in the intron 1 of the largemouth bass was identified with method of CRS-PCR. In addition, it was described a web-based program that facilitates the design of mismatched PCR primers to create or remove a restriction endonuclease recognition site and considerations for CRS-PCR primer design. The web-based program advanced the application of CRS-PCR in aquatic molecular breeding.
出处
《水生态学杂志》
北大核心
2009年第5期144-148,共5页
Journal of Hydroecology
基金
广东省自然基金项目(7301732)
国家科技支撑项目(NO.2006BAD01A1209)
国家科技基础条件平台工作项目(2005DKA21103)
