摘要
本研究采用巢氏PCR、降落PCR技术扩增牛SLC11A1基因的内含子9和11,分别获得777、715bp的片段,利用DNA测序技术对这2片段测序,发现3个新SNPs,分别为内含子9的6067(A/G)、6358(C/T)和内含子11的7809(A/T)。同时利用CRS-PCR和PCR-RFLP方法对这3个SNPs进行基因型分型,分析944头中国荷斯坦牛、鲁西黄牛和渤海黑牛的SLC11A1基因的多态性。结果表明,SLC11A1基因的3个SNPs的AA基因型频率最高,优势等位基因均为A;适合性检验表明,6358(C/T)和7809(A/T)位点在中国荷斯坦牛与鲁西黄牛群体中已达到Hardy-Weinberg平衡状态(P>0.05),而6067(A/G)位点的突变在牛群中均未达到Hardy-Weinberg平衡状态(P<0.05);同时中国荷斯坦牛群体在这3个基因座位上的多态信息含量均大于鲁西黄牛与渤海黑牛。
Nest-PCR and touchdown PCR technique were used to amplify 777 bp and 715 bp fragments of intron 9 and 11 of solute carrier family 11 member 1（SLC11A1）gene,respectively.Sequencing results showed that three new single nucleotide polymorphisms （SNPs） were identified at position of 6067（A/G）,6358（C/T） and 7809（A/T） in the SLC11A1 gene intron9 and 10,respectively.The SNPs of SLC11A1 gene in China Holstein cattle,Luxi Yellow cattle and Bohai Black cattle （n=944） were genotyped by CRS-PCR and PCR-PFLP.The results show that three genotypes namely AA,BB,and AB were detected,and Allele A and genotype AA were predominant.Chi-square test indicated that 6358（C/T） and 7809（A/T） of China Holstein and Luxi Yellow were in accordance with the Hardy-Weinberg equilibrium （P0.05）,while 6067（A/G） polymorphic sites did not meet Hardy-Weinberg equilibrium （P0.05） in three different cattle.The value of polymorphism information content of three SNPs in China Holstein cattle was higher than Luxi Yellow cattle and Bohai Black cattle.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2010年第5期698-703,共6页
Chinese Journal of Veterinary Science
基金
国家高技术研究发展计划基金资助项目(2006AA10Z1D9
2007AA10Z169)
公益性行业科研专项基金资助项目(ny-hyzx07-036-09)
山东省良种工程基金资助项目(2006LZ10-04)
山东省科技攻关基金资助项目(2006GG2209011)
