摘要
目的建立免疫浊度法检测血浆心脏型脂肪酸结合蛋白,探讨心脏型脂肪酸结合蛋白在早期急性心肌梗塞中的诊断价值。方法建立免疫浊度法反应体系;应用本研究所建立的免浊度法疫观察早期急性心肌梗塞患者0～6 h血浆心脏型脂肪酸结合蛋白的变化情况,并与Sandwich-ELISA法进行比较;比较心脏型脂肪酸结合蛋白与肌红蛋白、肌酸激酶同工酶和肌钙蛋白T/I用于诊断早期急性心肌梗塞的灵敏度和特异性。结果免疫浊度法具有与Sandwich-ELISA法相似的检测效果,两者具有较好的相关性(r=0.966);早期急性心肌梗塞组血浆心脏型脂肪酸结合蛋白较对照组明显升高(P<0.05);心脏型脂肪酸结合蛋白具有较好的精密度、较高回收率和不易受其他因素干扰;与肌红蛋白、肌酸激酶同工酶和肌钙蛋白T/I相比,心脏型脂肪酸结合蛋白对早期急性心肌梗塞诊断具有较好的灵敏度和特异性。结论与ELISA法相比,免疫浊度法检测心脏型脂肪酸结合蛋白具有快速、简便、灵敏度和特异性高的特点,值得临床推广应用。
Objective To establish turbidity immunoassay on the analysis ofsera heart-type fatty acid binding protein （H-FABP） and to evaluate its value on the early acute myocardial infarction （AMI）. Methods To establish experimental parameters of turbidity immunoassay system and compare turbidity immunoassay with Sandwich-ELISA on the analysis of H-FABP in both control group and AMI group （0 - 6 h after onset）. To study sensitivity and specificity of H-FABP, MB, CK-MB and CTnT/I on the early diagnose value of AMI. Result The efficiency of turbidity immunoassay was similar with Sandwich-ELISA, and they had a well correlation coefficient （r=0.966）. H-FABP in AMI group was higher than that in the control group （P〈0.05）. H-FABP had a high accuracy and recovery rate, and interference analysis was not found. H-FABP has a higher sensitivity and specificity than MB, CK-MB and CTnT/I, H-FABP on the early diagnose of AMI. Conclusions Using turbidity immunoassay on detection H-FABP is quick and easy. It has high sensitivity and specificity characters, which deserves to be applied broadly.
出处
《临床医学工程》
2009年第9期3-5,共3页
Clinical Medicine & Engineering
基金
深圳市科技计划立项项目(编号:200703179)
关键词
急性心肌梗塞
免疫浊度法
心脏型脂肪酸结合蛋白
Acute Myocardial Infarction
Turbidity Immunoassay
Heart-type Fatty Acid Binding Protein
