摘要
背景:通过制备肝素化胶原/壳聚糖支架来提高与转化生长因子β1的结合率,将脂肪干细胞种植于该支架材料上后直接种植于体内,可避免体外诱导过程,缩短组织工程构建的时间。目的:探索结合有转化生长因子β1的肝素化胶原/壳聚糖支架与脂肪干细胞复合修复兔软骨缺损的可行性。设计、时间及地点:软骨组织工程体外和体内实验相结合的研究,于2007-09/2008-07在南京大学生命科学院和解放军南京军区南京总医院动物试验中心完成。材料:分离培养兔脂肪间充质干细胞,行体外扩增培养至第3代,达到一定数量后接种于结合有转化生长因子β1的肝素化胶原/壳聚糖支架上得到细胞支架复合物。方法:取30只新西兰大白兔制备兔膝全层软骨缺损模型。随机选取一侧植入细胞支架复合物(实验组),其中15只另一侧植入结合有转化生长因子β1的肝素化胶原/壳聚糖支架(单纯支架组),余15只另一侧不做任何处理,作为空白对照。主要观察指标:于12周取材,从大体和组织学方面观察软骨修复情况。结果:实验组缺损区大部分被修复,缺损区被软骨组织充填,组织学检查提示形成典型的透明样软骨结构。而单纯支架组多不完全充填,其本为纤维组织状物覆盖,组织学检查未见明显软骨修复。空白对照组无明显修复。结论:结合有转化生长因子β1的肝素化胶原/壳聚糖支架与脂肪干细胞复合能较好修复软骨缺损。
BACKGROUND：Heparinized collagen/chitosan scaffold was established in order to increase the binding rate of transforming growth factor β1 （TGF-β1）. Adipose-derived stem cells plated on this scaffold were implanted in vivo so as to avoid from in vitro induction and shorten time of tissue engineering construction.
OBJECTIVE ： To investigate the feasibility of TGF-β1-induced heparinized collagen/chitosan scaffold binding with adipose-derived stem cells to repair rabbit knee joint cartilage defect.
DESIGN,TIME AIND SETTING ： A cartilage tissue engineering study combined with in vivo and in vitro experiment was performed at College of Life Science, Nanjing University and Animal Experimental Center, Nanjing General Hospital of Nanjing Milltary Area Command of Chinese PLA from September 2007 to July 2008.
MATERIALS： Adipose-derived stem cells were isolated from adipose tissue of rabbits and amplified to the third generation. Thereafter, the cells were inoculated onto TGF-β1-induced heparinized collagen/chitosan scaffold.
METHODS： A total of 30 rabbits were collected to establish full-thickness knee joint cartilage defect models. Composite scaffold was randomly implanted into one side of all rabbits as experimental group. TGF-β-induced heparinized collagen/chitosan scaffold was implanted into the other side of half rabbits as scaffold group, and the remaining rabbits were not treated with any materials as blank control group.
MAIN OUTCOME MEASURES. At 12 weeks after operation, cartilage repairing was detected grossly and histologically.
RESULTS： The defect was generally repaired in the experimental group and filled by cartilage tissue. Histological examination demonstrated that typical clear cartilage structure was found. However, the defect was incompletely filled in the scaffold group, Le., the defect was covered by substance like fibrous tissue. Histological examination indicated that cartilage repairing was observed obviously. The defect in the blank control group was not repaired.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第34期6611-6616,共6页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
全军“十一五”重点攻关项目(06G043)~~
