摘要
应用超薄切片和电镜技术,详细观察了蜕皮激素(MH)对家蚕Bombyx mori后部丝腺细胞超微结构的影响。电镜观察表明,家蚕后部丝腺细胞对MH处理极为敏感。一经MH处理,其细胞核的形态结构和细胞质中各种细胞器的发生、发展都呈现出明显的变化,并且与MH处理时期及剂量有关:五龄前期适量MH(4μg/头)处理,能促进与丝蛋白合成有关细胞器的形成与发展,加速腺细胞的成熟生长;较高剂量(8—16μg/头)处理,则导致自噬体的过早发生。五龄中、后期MH处理,一方面促进了粗面内质网等细胞器的进一步发达,另一方面也提高了自噬体的发生数量;处理剂量越高,后一种倾向越突出。这些结果证明,后部丝腺细胞超微结构的变化受MH调节。
By means of electron microscopy, the effect of oral administration of 20-hydroxyecdy-sone (MH) on the ultrastructure of the posterior silk gland cells in the silkworm Bombyx mori was investigated The result showed that the posteruor silk gland cells were very sensitive to MH treatment and immediate conspicuous changes would appear in the shape and structure of the nuclei as well as the development of organelles in the cytoplasm. The changes differed with the time of treatment and the MH dosage. The treatment with medial dosage (4 (xg/larva) enhanced the formation and development of the organelles related to silk protein synthesis and promoted cellular growth, while the treatment with higher dosage (8-16 μg/ larva) would result in the earlier formation of lysosomes in the early fifth larval instar. MH treatment in the intermediate and late stages of the fifth instar could on one hand enhance further development of the rough endoplasmic reticulum and other organelles and on other hand bring forth the occurrence of more lysosomes; the higher the dosage the more lysosomes occurred. These results demonstrate the regulatory action of MH on the ultrastructural change of the posterior silk gland cells.
出处
《昆虫学报》
CAS
CSCD
北大核心
1990年第4期398-402,共5页
Acta Entomologica Sinica
关键词
家蚕
后部丝腺细胞
超微结果
MH
Bombyx mori--posterior silk gland cell--ultrastructure--20-.hydroxyeedysone
