摘要
目的:研究吗啡对大鼠急性心肌缺血再灌注(AMIR)损伤的保护作用及对血清肿瘤坏死因子-α(TNF-α),血浆内皮素-1(ET-1)浓度的影响,探讨吗啡对AMIR损伤的保护机制.方法:将SD大鼠40只随机分为4组:单纯AMIR组(A组),吗啡预处理组(B组),吗啡+纳络酮组(C组),正常对照组(D组),每组10只.实验动物采用戊巴比妥钠40mg/kg腹腔注射麻醉,开胸结扎左冠状动脉前降支(LAD)制备AMIR模型.应用放射免疫测定法测定血清TNF-α,血浆ET-1含量.处死大鼠后将心脏切成4mm厚切片,浸入2,3,5-氯化三苯基四氮唑(TTC)磷酸缓冲液染色,正常心肌染色呈砖红色;坏死心肌呈灰白色,数码相机拍照并用图像分析系统计算心肌梗死面积.结果:AMIR4.5h时A,C组血清TNF-α浓度高于B组,差异具有显著性[(0.78±0.21)vs(0.43±0.08),(0.76±0.16)vs(0.43±0.08),P<0.01].AMIR4.5h时A,C组血浆ET-1浓度较B组增高,差异具有显著性[(65.00±8.00)vs(38.00±7.00),(61.00±11.00)vs(38.00±7.00),P<0.01],而B组与D组浓度差异无显著性.B组与A,C组相比较心肌梗死范围缩小,差异具有显著性[(22±5)%vs(38±6)%,(22±5)%vs(36±4)%,P<0.01];而A,C组心肌梗死面积无显著差异.结论:吗啡预处理可抑制血清TNF-α,血浆ET-1的浓度,从而保护缺血再灌注对心肌细胞的损伤.
AIM： To observe the influence of morphine on serous TNF-α and plasmatic ET-1 concentration and to study its protective effect on acute myocardial ischemic-reperfusion （AMIR） injury in rats. METHODS： Forty SD rats were divided randomly into 4 groups ： ischemic-reperfusion group （ group A）, morphine preconditioning group （group B ） , morphine and naloxone group（group C）, and normal control group（ group D）. The animal model of AMIR was established in rats by tying the left anterior descending branch（LAD） of rat coronary for 30 rain and then untying for 4 h. The animals were then sacrificed and hearts were harvested for determination of myocardial infarct size by 2, 3, 5-triphenyltetrazolium chloride （TTC）. Radioimmunoassay was conducted to detect the tumor necrosis factor alpha（TNF-α） in serum and endothelin-1 （ ET-1 ） in plasma. RESULTS ： At 4.5 h of myocardial isehemic-reperfusion （MIR） , both TNF-ct concentration in serum and ET-1 concentration in plasma increased significantly in Group A and Group C compare with those in Group B （both P 〈 0.01 ）, but no significant difference was observed between Group B and Group D. The myocardium infarct size was markedly smaller in group B compared with that in Group A and Group C （P 〈 0. 01 ）, but no significant difference was found between Group A and Group C. CONCLUSION： Morphine exerts protective effects on acute myocardial ischemic （AMI） by decreasing serum TNF-α and plasmatic ET-1 concentration and shrinking myocardial infarct size.
出处
《第四军医大学学报》
北大核心
2009年第17期1584-1586,共3页
Journal of the Fourth Military Medical University
