摘要
目的观察大鼠骨髓间质干细胞（rMSCs）移植对缺氧／缺血性脑损伤的修复作用。方法从大鼠骨髓中分离rMSCs进行培养扩增。制备缺氧／缺血性脑损伤大鼠模型，制模后第3日进行经5-溴-2-脱氧尿嘧啶核苷（BrdU）标记的rMSCs脑内移植。移植后7、14、21、30d进行Y迷宫实验检测大鼠的学习和记忆保持能力；另取脑组织进行苏木素-伊红（HE）染色和免疫组化，观察rMSCs对大鼠脑损伤的修复情况及rMSCs在脑内的定位。结果Y迷宫实验发现rMSCs移植组在各个时间点学习和记忆保持能力都优于模型组和PBS对照组（P均〈0．01）。HE染色发现缺氧／缺血性脑损伤模型大鼠主要表现为皮质、海马神经元变性、坏死，神经胶质细胞增生，胶质瘢痕形成。rMSCs移植后损伤脑组织得到不同程度的修复，移植的rMSCs在脑内存活，并主要迁移到皮质、海马等损伤部位。结论rMSCs移植对缺氧／缺血性脑损伤大鼠模型的学习和记忆保持能力的恢复具有显著的促进作用；其作用与移植的rMSCs向脑损伤部位的迁移和植活有关。
Objective To observe the influence of transplantation of rat bone marrow mesenchymal stem cells （rMSCs） on repairing hypoxic/ischmic brain injury in rats. Methods rMSCs were separated from the rat bone marrow and cultured in culture medium to proliferate. The rat models of hypoxic/ischemic brain injury were set up. Three days after the establishment of models, rMSCs labeled by 5-bromo-2-deoxyuridine （BrdU） were transplanted into the brain of a group of rats, the transplanted group. At the day 7, 14, 21, 30 after the transplantation, the study and memory abilities of the rats were observed by Y-maze test. After the animals were sacrificed, their brains were examined by hematoxylin and eosin （HE） staining and the immunohistochemistry method to observe the recovery of injured brain cells and the locations of rMSCs in the brain. Results At every time point, the Y-maze test showed that the study and memory abilities maintained in the transplanted group were significantly better than those in the model and control groups （all P〈0.01）. The research of HE staining revealed that the pathological changes of rat hypoxic/isehemic brain damage were mainly neuron necrosis, neuroglia proliferation and scar formation in the areas of cortex and hippocampus. The injured brain tissues were repaired to a certain extent after the transplantation of rMSCs. The transplanted rMSCs could survive and migrate to the injury site especially to the cortex and hippocampus. Conclusion The transplantation of rMSCs may significantly improve the learning capacity and remarkably ameliorate memory deficits in rats with hypoxie/isehemie brain injury. The effect is due to the survival and migration of rMSCs to the injury site in the brain.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第1期34-37,66,共5页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家中医药管理局科研基金资助项目（国中医药科2003LHR11）
浙江省教育厅重点科研项目（20060709）
浙江省卫生厅科研基金资助项目（2006A092）
关键词
骨髓间质干细胞
移植
缺氧/缺血性脑损伤
大鼠
bone marrow mesenchymal stem cell
transplantation
hypoxic/ischemic brain injury
rat
