摘要
目的探讨脑梗死慢性期神经元轴突的损伤情况及银杏叶提取物（EGB761）的干预作用机制。方法用光化学法制备大脑皮质局灶缺血梗死（PCI）模型。将15只大鼠随机分为EGB761组、磷酸盐缓冲液（PBS）组、假手术组，每组5只。EGB761组腹腔注射25mg／kg的EGB761，假手术组、PBS组腹腔注射等量PBS，每日1次，共7d。PCI后1d用磁共振成像（MRI）技术观察梗死灶的情况并计算其体积。PCI后7d处死大鼠后取脑，电镜下观察梗死灶附近皮质神经元轴突的损伤情况。结果光化学法可以成功复制PCI模型。PBS组和EGB761组梗死灶体积分别为（265．1±24．2）mm^3和（262．9士26．3）mm^3，差异无统计学意义（P〉0．05）。PCI后7d，与假手术组比较，PBS组可见到严重的病变，无鞘纤维中可见广泛的轴浆水肿或溶解、基质变淡；有鞘纤维中可见广泛的髓鞘增厚或皱缩、板层紊乱。EGB761组则较PBS组的病变显著减轻。结论脑梗死发生后在较长的一段时间内都存在着显著的轴突损伤，而EGB761则对这种轴突损伤有显著的保护作用。
Objective To approach the neuronal axon injury situation during chronic stage of cerebral infarction and the interference mechanism of ginkgo biloba extract （EGB761,银杏叶提取物）. Methods The rat model of cerebral cortical focal ischemic infarction named as photo-thrombotic cortical injury （PCI） was induced by photochemistry. Fifteen Sprague-Dawley （SD） rats were divided into three groups （each n=5）： EGB761 group, phosphate buffer solution （PBS） group and sham operation group. EGB761 group was treated by intra-peritoneal injection of EGB761 （25 mg/kg）; equal amount of PBS was injected into the peritoneal cavity of sham operation and PBS groups, once everyday for 7 days. One day after the establishment of PCI, magnetic resonance imaging （MRI） was applied to investigate the infarction focus and calculate its volume. Seven days after PCI formation, the rats were sacrificed and their brains were taken. Under the electron transmission microscope, the situation of cortical neuronal axon injury in and close to the focus of infarction was studied. Results The cortical infarction in rat was successfully induced by photochemistry. The volumes of infarction area in EGB761 and PBS groups were similar [（265.1±24.2） mm^3 vs. （262.9±26.3） mm^3, P〉0. 053. Seven days after PCI formation, compared with sham operation group, the pathological changes in PBS groups were more serious, in the fiber without neurilemma, extensive edema or dissolution in axoplasm and paler matrix could be seen, and in the fiber with neurilemma, extensive sheath thickening or folding and shrinking with lamella arrangement disturbance could be found. The pathological changes of axons in EGB761 group were obviously lighter than those in PBS group. Conclusion In a relatively long time after the onset of cerebral infarction, significant axon injury exists; EGB761 has an obvious protective effect on this kind of axon injury.
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第3期149-151,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
广东省科技计划项目（2006836004010,20078031402005）
关键词
脑梗死
银杏叶提取物
轴突
cerebral infarction
ginkgo biloba extract
axon
