摘要
实验发现，牛血清白蛋白可以大大增强杀鼠剂溴鼠灵的荧光。利用荧光猝灭法考察了溴鼠灵和牛血清白蛋白的相互作用。结果表明溴鼠灵对牛血清白蛋白的内源荧光有较强的猝灭作用，两者形成了新的复合物，属于静态荧光猝灭。跟据荧光增敏现象，建立了水溶液中测定溴鼠灵的荧光方法。在优化实验条件下，线性范围为5．0×10^-8～4．0×10^-7mol／L和4．0×10^-7～1．5×10^-5mol／L，检出限为5．9×10^-9mol／L。该方法用于渠水中微量溴鼠灵的测定，回收率为94．1％～101．3％．
The significant enhancement of relative fluorescence intensity of brodifacoum, an anticoagulant rodenticide, by bovine serum albumin （BSA） was observed. The mutual interaction of BSA with brodifacoum was investigated using fluorescence quenching spectroscopy. It was proved that the fluorescence quenching of BSA by brodifacoum was the result of the formation of brodifacoum-BSA complex. And the quenching belonged to static fluorescence quenching. According to fluorescence enhancement phenomenon, a spectrofluorimetric method for the determination of brodifacoum in aqueous media was established with the linear ranges of 5.0×10^-8～4.0×10^-7mol/L and 4.0×10^-7～1.5×10^-5mol/L. The detection limit was 5.9 × 10^-9 mol/L. It was successfully applied to determining the trace amount of brodifacoum in conduit water and the recoveries were in the range of 94.1% to 101.3%.
出处
《分析试验室》
CAS
CSCD
北大核心
2009年第9期20-23,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金(20575076)
山西省高校科技研究开发基金(20051223)项目资助
关键词
牛血清白蛋白
溴鼠灵
荧光分析法
测定
Bovine serum albumin
Brodifacoum
Fluorescence spectroscopy
Determination
