摘要
目的：建立全血中氟鼠灵、溴鼠灵的高效液相色谱紫外检测方法。方法：全血样品经乙酸乙酯提取后，采用XDB C18柱（150mm×2．1mm×5mm）；流速：500μl／min；柱温：30℃；流动相：甲醇＋0．2％乙酸（88＋12，v／v）；紫外检测器：270nm，外标法定量。结果：两种化合物在0．2～10．0mg／L范围内呈良好线性关系，氟鼠灵和溴鼠灵的回收率分别为87.6％和91．9％，RSD分别为6．3％和6．1％，最低定量检出限分别为10．1和2．6ng。结论：本方法简便、灵敏、重现性较好，为氟鼠灵和溴鼠灵中毒病人的诊断提供正确依据。
Objective：To develop an analytical method for the determination of flocoumafen and brodifacoum in whole blood by high - perfomance liquid chromatography with ultraviolet detection. Methods： After flocoumafen and brodifacoum in whole blood were extracted by ethyl acetate, the samples were separated on ZORBAX Eclipse XDB C18（ 150 mm ×2. 1 mm ×5 μm） by using the mobile phase consisting of CH3OH （88%） and 0. 2% CH3COOH （ 12% ） and detected by UV at 270 nm. The flow rate was 500 vd/min and the column temperature was controlled at 30℃. Results： Calibration curves were linear within the range of 0. 2 - 10. 0 mg/L and the limits of quantification were found to be 10. 1 ng and 2. 6 ng for flocoumafen and brodifacoum, respectively. The extraction recoveries were 87. 6% and 91.9% and the RSD were 6. 3% and 6. 1% for flocoumafen and brodifacoum, respectively. Conclusion：This method is found to be simple, sensitive, accurate and of little interference for the determination of trace flocoumafen and brodifacoum in poisoned patients.
出处
《中国卫生检验杂志》
CAS
2007年第1期13-14,93,共3页
Chinese Journal of Health Laboratory Technology
基金
宁波市医学科技计划项目(2004056)
