摘要
目的:通过免疫组化方法,研究肾上腺皮质分泌物嗜铬蛋白A(CgA)、神经特异性烯醇化酶(NSE)和突触素(SYN)在肾上腺皮质的分布特点,为进一步深入研究皮髓质之间的相互作用奠定基础。方法:运用免疫组化方法对肾上腺髓质增生、嗜铬细胞瘤和嗜铬细胞癌56份组织标本进行CgA、NSE和SYN染色,以正常肾上腺作为对照,观察其在皮质中的分布特点。结果:肾上腺嗜铬细胞瘤和嗜铬细胞癌的皮质中均可见CgA染色阳性细胞,髓质增生和正常肾上腺病例皮质CgA染色阴性;肾上腺髓质增生和嗜铬细胞癌皮质NSE染色呈阳性,嗜铬细胞瘤和正常肾上腺皮质NSE染色呈阴性;肾上腺髓质增生、嗜铬细胞瘤的皮质中SYN染色呈阳性,正常肾上腺嗜铬细胞癌皮质SYN染色呈阴性。结论:①肾上腺皮质CgA、NSE和SYN分布特点可以帮助鉴别肾上腺髓质增生、嗜铬细胞瘤和嗜铬细胞癌的异同;②肾上腺皮髓质之间联系紧密,受共同机制所调控,除经典的下丘脑-垂体-肾上腺轴和肾素-血管紧张素-醛固酮系统外,肾上腺皮髓质之间存在相互调节的解剖学和内分泌学基础。
Objective ： To study the characteristics of chromogranin （ CgA）, synaptophysin （SYN） and neuron-specific enolase （NSE） in the cortex of adrenal gland. Methods：Immunohistochemical technique was used to detect the expressions of CgA, SYN and NSE in 56 routinely processed tissue speci-mens from human adrenal cortical neoplasm and hyperplasia. Results： CgA immunoreactivity was regularly detected in the cortex of adrenal neoplasm, but not in the hyperplasia and normal adrenal gland. The immunoreactive materials appeared in the cytoplasm and in the form of vacuole or grains. Adrenal cortical neoplasm and hyperplasia showed NSE positive cells in the cortex, but not in the normal adrenal gland. SYN positive materials were shown in the cortex of hyperplasia and adrenocorticoadenoma, but not in the adrenocorticoadenocarcinoma and normal adrenal gland. Conclusion： The distributive characteristics of CgA, SYN and NSE in the adrenal cortex help the differential diagnosis of adrenal hyperplasia. The adrenal cortex is closely connected with medulla. Besides the classical hypothalamus-pituitary-adrehal axis （HPAA） and rennin-angiotensin-aldosterone system （RAAS）, the adrenal cortex and medulla are mutually regulated on the basis of anatomy and endocrinology.
出处
《医学研究生学报》
CAS
2009年第8期808-810,I0001,共4页
Journal of Medical Postgraduates
基金
广东省科技计划基金资助项目(批准号:2005B2570020)
