摘要
在利用木质纤维素稀酸水解液发酵生产微生物油脂的过程中,水解液须经过脱毒处理,以除去其中抑制菌体生长的有毒物质。采用活性炭对甘蔗渣稀酸水解液进行吸附脱毒,通过响应面中心复合设计(CCD)试验,研究了不同因素对活性炭脱毒的影响,并优化了活性炭脱毒的条件。在活性炭用量为3%(质量分数),温度为80℃,pH=2的条件下脱毒50 min,可以除去超过90%的酸溶木素及木素降解产物。用Ca(OH)2中和水解液后作为碳源,在限氮条件下采用圆红冬孢酵母菌(Rhodosporidium toruloides AS 2.1389)发酵生产油脂。结果表明,脱毒后的水解液可以很好地用于菌体生物量合成,其油脂含量为32.99%,油脂得率系数(油脂/糖)为12.48 g/100 g。
In the process of yeast oil production from lignocellulosic biomss, the acid hydrolyzate needs to be detoxified in order to remove the toxic compounds which inhibit the yeast growth. In this paper, the effects of several factors on activated charcoal detoxification of sugarcane bagasse hydrolyzate were investigated and the conditions of detoxification were optimized by response surface methodology （central composite design, CCD）. It was found that when the hydrolyzate were detoxified with 3% activated charcoal （w/w） at a temperature of 80 ℃ and at a pH value of 2.0 for 50 min, more than 90% of the lignin decomposition products were removed. Being neutralized by Ga （OH）2, the detoxified hydrolyzate was further used as carbon source for yeast lipids fermentation under nitrogen-limited conditions by Rhodosporidium toruloides AS2.1389. It was observed that the detoxified hydrolyzate could be well utilized by the yeast to synthesize biomass, and the lipids content reached to 32.99%（w/w） based on dry cell weight, with lipid yield of 12.48 g lipid/100 g sugar.
出处
《可再生能源》
CAS
北大核心
2009年第4期32-36,共5页
Renewable Energy Resources
关键词
木质纤维素
活性炭
脱毒
酵母油脂
木素降解产物
lignocellulose
activated charcoal
detoxification
yeast lipids
hgnin degradation products
