摘要
目的:观察大鼠失血性休克复苏后肠黏膜损伤修复的形态学特征与肠道细菌移位的关系。方法:将雄性SD大鼠42只随机分为对照组(n=7)和休克组(n=35),休克组又分为复苏后0、3、6、12和24h5个亚组,每个亚组均7只。建立大鼠肠缺血-再灌注模型,通过光镜观察不同时段回肠黏膜的改变及肝、肠系膜淋巴结和肾匀浆细菌培养。结果:休克组从复苏0h起回肠黏膜上皮发生损伤改变,6h大部分黏膜已修复,24h肠黏膜结构恢复正常;对照组大鼠肝、肠系膜淋巴结和肾匀浆平均组织含菌量与休克组比较差异均有统计学意义(P<0.05);休克组各亚组细菌培养除0h和24h,以及3h和6h外,其余亚组间比较差异均有统计学意义(P<0.05)。结论:失血性休克致肠缺血-再灌注损伤从而导致肠黏膜屏障受累,小肠黏膜通透性增加,引起细菌移位。
Objective： To investigate the relationship of morphological changes and bacteria translocation in small intestine mucosa after hemorrhagic shock in rats. Methods： Forty-two rats were randomly divided into 2 groups, control group （n=7）and shock group （n=35）. The shock group was divided into 0, 3, 6, 12 and 24 h five subgroups. The morphological changes in intestinal mucosa were observed under light microscope, including the histology of intestinal mucosa, determination of height of villiand evaluation of mucosa damage index in the different phases after traumatic hemorrhagic shock. The node of mesentery, liver and kidney were taken out in aseptic condition which were used to cell culture. Results： In shock group, mucosa epithelial injury was obvious in small intestine even at 0 hour. Most of the injured mucosa began to repair after 6 hours, and completed in 24 hours. The bacterial cultures were positive in the liver, node of mesentery and kidney in shock group, which were much higher than that in control group （P 〈 0.05）. There were statistical significances between shock subgroups except for 0 h, 24 h, 3 h and 6 h groups （P 〈 0.05 ）. Conclusion： In the early phase after hemorrhagic shock, intestinal mucosal barrier was subjected to damage, in which bacteria translocation was caused by the increasing permeability of small intestine.
出处
《天津医药》
CAS
北大核心
2009年第9期765-767,共3页
Tianjin Medical Journal
基金
河北省科技攻关基金资助项目(项目编号:05276101D-21)
