摘要
目的研究体重监测下渐进性口服二乙基亚硝胺(diethylnitrosamine,DENA)诱导大鼠肝硬变的效果和可行性。方法50只6周龄Wistar大鼠被随机分为3组:正常对照组(n=10)、普通DENA诱导组(n=20)和渐进DENA诱导组(n=20),观察诱导4周和8周后大鼠体重、死亡率、肝硬变形成程度等指标。结果肝硬变诱导4周后,普通DENA诱导组的体重为(234.9±27.1)g,显著低于正常对照组的(264.8±33.7)g(P<0.05);肝硬变诱导8周后普通DENA诱导组的体重为(251.5±34.3)g,显著低于正常对照组的(303.2±49.4)g(P<0.01)和渐进DENA诱导组的(277.5±27.6)g(P<0.05);正常对照组和渐进DENA诱导组体重比较的差异无统计学意义(P>0.05);正常对照组和渐进DENA诱导组大鼠均无死亡,与普通DENA诱导组(死亡率为35%)比较差异有统计学意义(P<0.05);普通DENA诱导组和渐进DENA诱导组的肝硬变形成率均为100%。结论口服DENA诱导肝硬变是一个简单、可复制和仿真性强的模型制作方法,体重监测下渐进性口服DENA的方法可有效提高大鼠肝硬变诱导过程的安全性,减少大鼠死亡。
Objective To study the effect and feasibility of gradual oral diethylnitrosamine （DENA） induced liver cirrhotic model in rats under avoirdupois monitoring. Methods Fifty Wistar rats （6 weeks old） were divided into 3 groups., normal control group （n= 10）, traditional DENA induction group （receiving traditional oral DENA treatment, n= 20）, gradual DENA induction group （receiving gradual oral DENA treatment under avoirdupois monitoring, n=20）. The weight, mortality and liver cirrhosis formation were observed. Results After 4 weeks of inducing cirrhosis, the weight of traditional DENA induction group [（234.9 ±27. 1） g] was significantly lower than that of normal control group [（264.8±33.7） g, P〈0.05]. After 8 weeks of inducing cirrhosis, the weight of traditional DENA induction group [（251. 5 ± 34. 3） g] was significantly lower than that of normal control group [（303.2±49.4） g, P〈0.01] and gradual DENA induction group [（277. 5±27. 6） g, P〈0. 05]. However, the difference between normal control group and gradual DENA induction group was not remarkable （P〉0.05）. The mortality in traditional DENA induction group （35 % ） was significantly higher than that in normal control group （0） and gradual DENA induction group （0）, P〈0.05. But the rate of cirrhosis formation both in traditional DENA induction group and gradual DENA induction group was 100%. Conclusion Oral DENA induced cirrhotic model in rats is a simple, reproducible and reliable technique. Gradual oral technique, in which DENA is given under avoirdupois monitoring, can improve rat＇s security and reduce mortality.
出处
《中国普外基础与临床杂志》
CAS
2009年第8期625-627,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
肝硬变
动物模型
二乙基亚硝胺
Liver cirrhosis
Animal model
Diethylnitrosamine
