摘要
利用分子标记技术AFLP标记,从15对引物中选取扩增效果较好的11对引物,对11只极端多胎个体和6只极端非多胎个体DNA进行扩增,共扩增出766个条带,平均多态率5.36%,其中引物E42/T48中1条294bp的条带在极端非多胎个体中均出现,而在极端多胎个体中只在1个个体中扩增出来,表现出极显著的差异。对差异片段回收、克隆、测序,设计4对引物,对基因组扩增后发现,产生差异片段的原因是片段内部有较长序列的插入或丢失,产生310bp和265bp2个等位基因,其中310bp纯合等位基因的初产羔羊数显著高于310bp和265bp杂合等位基因的初产羔羊数,在大足黑山羊地方类群内可能作为多胎性状分子标记。
A total of 15 pairs of AFLP primer combinations were used to search the differential fragment of gemomic DNA from 17 goats including 11 with extreme high fecundity traits and 6 with extreme low fecundity traits. The results showed that a total of 766 AFLP markers were obtained, the rate of polymorphisms was 5.36%. In primer combination E42/T48, a fragment of 294 bp was found in all 6 goats with extreme low fecundity traits while only in one goat with extreme high fecundity traits. Four pairs of primers were designed by reclaiming, cloning and sequencing with the differential fragment.After PCR with genome, it was proved that the reason producing differential fragment was insert or lose of a long sequence between the two primers. Both alleles of 310 bp and 265 bp long existed. The allele 310 bp was positively correlated with the first litter size evidently. That may become the molecullar marker with high fecundity traits of DBGP.
出处
《中国畜牧杂志》
CAS
北大核心
2009年第15期1-4,共4页
Chinese Journal of Animal Science
关键词
多胎
AFLP
差异片段
大足黑山羊
high fecundity
AFLP
differential fragment
Dazu black goat populations
