摘要
目的研究双顺反子逆转录病毒载体介导IL-2基因转导成纤维细胞对小鼠肝癌的治疗作用。方法利用双顺反子逆转录病毒载体pGCEN/IL-2将IL-2基因转导小鼠成纤维细胞NIH3T3,然后将分泌IL-2的成纤维细胞与60Gyγ射线照射的肝癌H22细胞皮下植入三天前5×105或2.2×105肝癌H22细胞皮下注射的BALB/c鼠。结果分泌IL-2的成纤维细胞治疗组能明显增加荷瘤小鼠的生存率(P<0.025),抑制肿瘤生长(P<0.05),在肿瘤植入第70天无肿瘤形成的小鼠再次注射1×106H22细胞仍然不发生肿瘤。51Cr释放试验表明来自照射H22与分泌IL-2成纤维细胞混合液注射的小鼠脾细胞与51Cr标记的H22细胞共培养后,51Cr特异释放明显高于对照组(P<0.05)。结论实验结果表明IL-2基因修饰的成纤维细胞能诱导针对肝癌的特异免疫反应。
Objective Study on antitumor effect of IL 2 gene modified fibroblasts by using bicistronic retrovirus vector. Methods The interleukin 2(IL 2) gene was introduced into mouse fibroblasts NIH3T3 with a bicistronic retroviral vector pGCEN/IL 2.The IL 2 secreting cells were then co transplanted with 60 Gy γ irradiated hepatoma H22 cells into syngeneic BALB/c mice that had been subcutaneously injected 5×10 5 or 2.5×10 5 H22 cells three days before. Results The period of survival of mice with 2.5×10 5 H22 cells treated with IL 2 secreting fibroblasts was significantly prolonged ( P <0.05)related to the mice receiving irradiated H22 cells alone or mice treated with nonsecreting fibroblasts.Tumor progression in the treated mice was significantly inhibited( P <0.001)and the mice without the occur of tumor were still tumor free when they were rechallenged with 10 6 H22 cells at day 70 after tumor implantation.Using a 51 [KG*2〗Cr release assay, the specific release of isotope from labeled H22 cells co incubated with the spleen from mice injected with the irradiated H22 cells IL 2 secreting fibroblasts were higher( P <0.05) than the control groups. Conclusion These results suggest that IL 2 modified fibroblasts may induce a specific immune response to hepatoma cells.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1998年第4期278-281,共4页
Chinese Journal of Microbiology and Immunology
基金
863高科技
国家自然科学基金
关键词
白细胞介素
成纤维细胞
基因疗法
肝肿瘤
Interleukin 2 Retrovirus Fibroblasts Gene therapy Neoplasms,experiment