摘要
目的观察结直肠癌患者围手术期外周血 T 细胞亚群,即所有 T 细胞的表面分子(CD3)、辅助 T 细胞表面分子(CDg)、抑制 T 细胞表面分子(CD8),肿瘤坏死因子-α(TNF-α)和可溶性白细胞介素-2受体(SIL-2R)表达的动态变化。方法应用碱性磷酸酶-抗碱性磷酸酶法和酶免疫法动态检测了60例结直肠癌患者围手术期外周血 T 细胞亚群、TNF-α和 SIL-2R 的表达。结果结直肠癌患者术前 CD3、CD4细胞明显减少,CD4/CD8比值明显降低,CD8细胞明显增加,TNF-α和 SIL-2R 水平明显高于对照组;术后随时间延长,CD3、CD4细胞及 CD4/CD8比值逐渐增加,CD8细胞、TNF-α和 SIL-2R 水平逐渐降低。结论结直肠癌患者免疫功能低下,T 细胞功能受到抑制,TNF-α及 SIL-2R 水平的异常表达与肿瘤负荷有明显关系,切除肿瘤可改善患者的免疫功能。
Objective The dynamic change of peripheral T cell subgroup and expression of TNF-α and SIL-2R was e- valuated in colorectal cancer patients during the perioperative period.Method APAAP and ELISA were used to measure the parameters in 60 cases.Result It was found that CD3 cell and CD4 cell decreased markedly preoperatively and the CD4/CD8 value declined.CD8 cell increased apparently.The levels of TNF-α and SIL- 2R were higher than that of control group.Postoperative CD3、CD4 cell and CD4/CD8 value increased gradual- ly,while CD8 cell、TNF-α and SIL-2R levels decreased accordingly.Conclusion Depressed immunity and T- Cell function were observed in colorectal cancer patients.The abnormal expression of TNF-α and SIL-2R was found to be related to the tumor preponderance,hence both radical and palliative resection improved the pa- tients' immunity.
出处
《中华普通外科杂志》
CSCD
1998年第4期207-20,共1页
Chinese Journal of General Surgery
