摘要
【目的】探讨抗坏血酸氧化酶(AAO)活性变化与西葫芦生长发育的关系,揭示不同蔓性西葫芦长势和产量存在差异的原因,为西葫芦的遗传育种研究提供参考。【方法】以2个半矮蔓西葫芦品种和2个矮蔓西葫芦品种为材料,采用完全随机区组排列,测定西葫芦叶片、果实生长发育的相关指标及AAO活性,研究AAO活性与西葫芦叶片及果实发育的关系。【结果】在西葫芦生长发育过程中,2个半矮蔓西葫芦品种C1、C2的叶长、叶宽以及果实瓜长、瓜径均较2个矮蔓西葫芦品种D1、D2大;而伴随着叶片与果实的发育,品种C1、C2叶片与果实中AAO活性均较品种D1、D2高,说明AAO活性与西葫芦叶片、果实的发育密切相关;在收获期,品种C1、C2产量较品种D1、D2大。4个西葫芦品种的不同叶龄AAO活性均存在差异,其活性依次为:顶端发育叶片>中部发育叶片>下层衰老叶片,说明AAO可能参与叶片的抗衰老功能。【结论】AAO活性与西葫芦的生长发育呈正相关,对西葫芦的生长发育及产量形成有促进作用。
[Objective] By searching for the relations between AAO and the development of Cucurbita pepo L. we wanted to find the reasons for the development and yield of different Cucurbita pepo L. varieties and give the references to the genetic and breeding research of Cucurbita pepo L. [Method] Two semibush varieties and two bush varieties were chosen to be materials which were completely randomly designed and three triplications. By determining the correlation indicators of the development of the leaf and fruit and the AAO activity,the relations between AAO and the development of the leaf and fruit of Cucurbita pepo L. were analyzed. [Result] The leaf length,leaf width,fruit length and fruit diameter of the two semi-bush varieties C1,C2 were all higher than those of the bush varieties D1,D2 during the development of Cucurbita pepo L. ;the AAO activity of the leaf and the fruit of variety C1,C2 was all higher than that of variety D1 ,D2 during the development of the leaf and the fruit, which indicated that AAO activity was correlated with the leaf and the fruit development ; at the mature stage, the yield of variety C1, C2 was higher than that of variety D1, D2. And AAO specific activity of different leaf ages among different varieties showed difference, the order being： Young〉intermediate〉old, which indicated that AAO may be correlated with anti-senescence function. [Conclusion] The AAO activity was correlated with development and yield of Cucurbita pepo L. ,which can promote the development and yield of Cucurbita pepo L,.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2009年第7期91-96,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
山西省科技攻关项目"优质抗病彩色西葫芦新品种选育"(20070310027-2)
关键词
西葫芦
叶片发育
果实发育
抗坏血酸氧化酶
Cucurbita pepo L.
development of leaf
development of fruit
ascorbic acid oxidase
