摘要
采用２，４二硝基氟苯作为衍生化试剂，１６种常见人体生理性氨基酸为分析对象，区带毛细管电泳为分离模式，建立一种新的氨基酸衍生化方法。系统研究了反应时间、反应温度和ｐＨ值对衍生化反应的影响。同时观察了４℃和２５℃室温下衍生化产物的稳定性情况，结果发现，无论是在４℃冰箱，还是在２５℃室温下，氨基酸衍生化产物保存５天，紫外吸收值无明显改变。方法的性能指标测定表明，该法的线性在１０～７００μｍｏ１／Ｌ范围内良好（γ在０．９６４～０．９９５之间）。最低检测限（Ｓ／Ｎ＝２）为２．５～７．９μｍｏｌ／Ｌ。测得平均回收率在８６．３～１０７．４％之间。该法具有衍生化反应操作简便、灵敏度高、稳定性好、线性及回收率佳等特点，不仅可用于氨基酸的衍生化反应，而且同样适合于其它含有氨基的生物活性物质的衍生化反应。
novel derivative method for the determination of amino acids was developed by the use of 2,4 dinitrofluorobenzen.16 kinds of amino acids were analyzed with high performance capillary electrophoresis.The conditions of derivatization were studied to find an optimal procedure,which included the reaction time,reaction temperature and pH value.The observation of stability shows the products of derivatization of amino acids stay steady for five days at 4℃ or 25℃.The linearity of this assay was 10～700μmol/L,and the relative coefficients were from 0.964 to 0.995.The minimal detection limit(S/N=2) was 2.5～7.9μmol/L.The average recoveries were from 86.3 to 107.4%.The method has many advantages such as rapidity,accuracy,simplicity,sensitivity and stability,which suits to the derivatization of amino acids and other substances which have amino group.
出处
《基础医学与临床》
CSCD
1998年第4期75-80,共6页
Basic and Clinical Medicine
关键词
氨基酸
衍生化
2.4-二硝基氟苯
毛细管电泳
Amino acids Derivatization 2,4 Dinitrofluorobenzen Capillary electrophoresis
