摘要
目的探讨不同海拔高度严重烧伤延迟复苏大鼠脑组织氨基酸变化及其意义。方法雄性Wistar大鼠120只,在海拔3800M与海拔1517M制作实验动物模型(TBSA30%,Ⅲ度),并随机分为延迟、即时复苏组和正常对照组,分别于伤后1h、6h、12h、24h、72h、7d取材。应用高效毛细管电泳法检测脑组织中兴奋性氨基酸(谷氨酸)和抑制性氨基酸(甘氨酸、γ-氨基丁酸)含量。结果高原延迟复苏组(HDFR)兴奋性氨基酸(谷氨酸)伤后较对照组(HNC)和即时复苏组(HIFR)显著降低,抑制性氨基酸(γ-氨基丁酸、甘氨酸)较HNC组、HIFR组显著升高。HIFR组伤后3d基本恢复至对照水平,HDFR组伤后7d,尚未恢复正常。高原地区与兰州地区各时相点比较兴奋性氨基酸降低,抑制性氨基酸升高,兰州地区即时复苏组(LIFR)伤后12h～24h基本恢复正常,兰州地区延迟复苏组(LDFR)伤后72h恢复正常。结论高原地区严重烧伤延迟复苏大鼠脑组织中兴奋性氨基酸增多,抑制性氨基酸减少,此变化加重了脑损伤。
Objective To explore the changes and significance of cerebral amino acid with delayed fluid resuscitation after severe burn in rats at different altitudes.Methods 120 male Wistar rats were used to establish the experimental models (TBSA 30%,Ⅲo) at high altitude,and they were randomly divided into 3 groups: delayed fluid resuscitation group (HDFR),immediately fluid resuscitation group (HIFR) and normal control group (HNC). The samples were harvested at 1 h,6 h,12 h,24 h,72 h and 7 d respectively. The same experiment was repeated in Lanzhou area. The changes of Glu,GABA and Gly in cerebral tissues were detected by means of high performance capillary electrophoresis (HPCE).Results There were obvious decrement of excitatory amino acid (Glu) and increment of inhibitory amino acid (GABA and Gly) in HDFR comparing with HNC and HIFR.They returned to the control level on the third day post burn in HIFR,but they did not return at 7 d in HDFR.Compared with each group in Lanzhou area,the content of excitatory amino acid (Glu) was decreased and inhibitory amino acid (GABA and Gly) was increased. They returned to the control level at 12 h-24 h post burn in LIFR and returned at 72 h in LDFR.Conclusion The changes of excitatory amino acid decrement and inhibitory amino acid increment might aggravate to the cerebral injury in burned rat at different altitudes.
出处
《兰州大学学报（医学版）》
CAS
2005年第1期1-4,共4页
Journal of Lanzhou University（Medical Sciences）
