摘要
利用PCR-RFLP方法检测TLR2基因在297头中国荷斯坦牛中的多态性,并分析其多态性与体细胞评分(SCS)的相关性。结果显示,TLR2基因exon2扩增片断的109 bp处产生C→A的突变,并导致天冬氨酸改变为谷氨酸。TLR2基因exon2被EcoRⅤ消化后表现多态性,表现AA、AB、BB 3种基因型,其中B等位基因为优势等位基因,等位基因频率为0.784 5,而A等位基因频率则为0.215 5。经χ2适合性检验,中国荷斯坦牛在该位点达到Hardy-Weinberg平衡状态。同时,群体EcoRⅤ基因座不同基因型与SCS相关分析的结果表明,BB?AB型个体的SCS指标显著高于AA型(P<0.05),AA基因型可作为乳腺炎抗性的有利基因型,可将TLR2作为奶牛乳腺炎候选基因,应用于乳腺炎抗性分子标记辅助选择育种。
In this research, polymorphisms of TLR2 gene in Chinese Holstein（n= 297） were detected with PCR- RFLP,and relationship between polymorphisms of TLRZ and somatic cell score （SCS） was analyzed. Results showed that a polymorphic site was identified,where there was a mutation C→A at position 106 bp, which resulted in Asp replaced by Glu. Genotype AA, AB and BB were detected by digesting the fragment with restriction endonuclease EeoRV in the population. B allele was predominant and allele frequency A and B were estimated as 0. 784 5, 0. 215 5. ;g2 test indicating that the polymorphism locus in Chinese Holstein fitted Hardy-Weinberg equilibrium（P〈 0.05）. In addition, the effect of the TLR2 polymorphism on SCS was analyzed. Results indicated that the SCS of BB and AB genotype cows was significantly higher than AA genotype （P〈0.05）. AA genotype was beneficial to masti- tis resistance. We concluded that TLR2 gene could be a candidate gene of mastitis resistance for molecule marker-assisted selection breeding. This study offers a theoretical basis on selection of the mastitis resistance in Holstein cattle.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第8期1065-1068,共4页
Chinese Journal of Veterinary Science
基金
国家"863"计划资助项目(2006AA1021D9
2007AA-10Z169)
山东省农业科学院高技术创新基金资助项目(2006YCX028)
