摘要
目的:利用种植型牵张器增高兔下颌牙槽嵴,研究一氧化氮合酶(NOS)与转化生长因子-β1(TGF-β1)在牵张成骨过程中的相关性。方法:雄性日本大耳白兔24只,随机分为牵张术后1天、1周、2周、4周、6周组和空白对照组,每组4只。随机选取一侧下颌骨行牵张成骨术。分离处死后的兔下颌骨,行免疫组化观察。对免疫组化染色切片通过高清晰彩色病理图像分析仪测量灰度值,计算染色强度。利用SPSS13.0软件包进行方差分析和Spearman相关分析,并拟合曲线关系,建立相关回归方程。结果:在牵张成骨中,eNOS与TGF-β1呈正相关(P<0.01,r=0.538),iNOS与TGF-β1不存在相关性(P>0.05,r=0.283);eNOS与TGF-β1的曲线拟合回归方程为YeNOS=24.246-1.914XTGF-β1+0.182XTGF-β12-0.004XTGF-β13。结论:TGF-β1在牵张成骨过程中可能起调控作用,对eNOS起正性调控作用,通过调控eNOS来完成对iNOS的调控,iNOS又通过NO浓度的变化完成对TGF-β1的反作用。
PURPOSE： To analyze the correlation of nitric oxide synthase and transforming growth factor 131 in distraction osteogenesis, METHODS： 24 male rabbits were randomly divided into 6 groups, each group comprising 4 rabbits. Distraction osteogenesis was carried out on the random side of the alveolar bone. After 4 days of latent period, the alveolar bone was vertically distracted at a rate of 1.0 mm/d and rhythms of 2 times/day for 4 days by implant-distraction. Staining strength of NOS and TGF-β1 in immunohistochemistry were analyzed using SPSS 13.0 software package at the point of 1 day, 1 week, 2 weeks, 4 weeks and 6 weeks of DO, RESULTS：The eNOS were positively correlated with TGF-β1 （P〈 0.01, r=0.538）, iNOS and TGF-β1 had no correlation （P〉0.05, r=0,283）; the regression equation of eNOS and TGF-β1 was YeNOS=24.246--1.914XTGF-β1＋0.182XTGF-β1^2--0.004XTGF-β1^3. CONCLUSIONS：The TGF-β1 adjust iNOS by control of eNOS, eNOS and iNOS also have a counteraction to TGF-β1 by controlling NO.
出处
《中国口腔颌面外科杂志》
CAS
2009年第4期356-360,共5页
China Journal of Oral and Maxillofacial Surgery
关键词
牵张成骨
一氧化氮合酶
转化生长因子-Β1
免疫组化
兔
Distraction osteogenesis
Nitric oxide synthase
Transforming growth factor β1
immunohistochemistry
Rabbit
